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1. Introduction
2. Barrier mechanisms in the developing brain
3. The brain barriers - overview
4. Claude Bernard - the brain's internal environment
5. Alcian blue stain of gut, eye and brain
6. Paul Ehrlich
7. Edwin Goldmann
8. Max Heinrich Lewandowsky
9. Tight junctions and their function in the BBB
10. Hugh Daveson
11. The brain barriers in the embryo
12. Outline
13. Brain development - different species
14. Trypan blue dye of fetal guinea pig
15. Dye injection to the CSF, Pig 9mm CRL embryo
16. Stern - Dye injection in newborn animals
17. Newborn mouse injected with Trypan blue
18. Colloids and crystalloids dyes
19. Freeze fractures in brain development
20. Freeze fractures of blood vessel tight junctions
21. Tight junctions permeability in developing brain
22. Biotin labeled molecules for permeability testing
23. Junctions are functionally tight in early stages (1)
24. Junctions are functionally tight in early stages (2)
25. Junctions are functionally tight in early stages (3)
26. A possible trans-cellular route of entry
27. Tightness of blood vessels in developing brain
28. P1 Monodelphis, stained with anti-albumin
29. Mechanisms of barriers induction
30. Molecular basis of induction process
31. Radio-labeled permeability markers experiments
32. The sheep placenta
33. Marker levels after IV injection - plasma and CSF
34. CSF:plasma ratio
35. Experiments with marsupial new born
36. Brain development of a newborn opossum
37. CSF:plasma ratio in opossum (1)
38. Brain:plasma ratio in opossum
39. CSF turnover
40. CSF secretion in the developing brain
41. CSF:plasma ratio in opossum (2)
42. Establishment of ion gradients CSF:plasma
43. Ion gradient in brain development
44. Chloride gradient in rats
45. Effective barrier mechanisms in developing brain
46. Adult ion transfer
47. Transporter expression in embryo choroid plexus
48. AQP1 - lateral ventricle (rat)
49. Lateral ventricular volume
50. High concentration of proteins in CSF
51. Protein concentration in CSF (rat)
52. High protein levels exert an osmotic effect
53. Strap junctions
54. Freeze fractures of strap and tight junctions
55. TEM of strap and tight junctions
56. Protein positive epithelial cells, LV choroid plexus
57. P6 M. domestica: albumin in choroid plexus
58. Expansion of the ventricular system
59. Summary
60. Acknowledgements
61. References

Topics Covered

• The brain barriers
• Historical perspective
• Morphology of cerebral blood vessels and choroid plexus in the development brain
• Are tight junctions functionally tight?
• Ion and water transfer mechanisms
• Origin and significance of high CSF protein concentration in embryos
• CSF-brain barrier in the developing brain

Links

Series:

• The Blood-Brain Barrier

Categories:

• Neuroscience

Therapeutic Areas:

• Neurology

Talk Citation

Saunders, N. (2009, November 19). Barrier mechanisms in the developing brain: mechanisms and misunderstandings [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved April 15, 2025, from https://doi.org/10.69645/XZBH4186.
Export Citation (RIS)

Publication History

• Published on November 19, 2009
• Archived on May 31, 2018

Financial Disclosures

• Prof. Norman Saunders has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.