• View the Talks
• 37 min
  1. Copy number variation

  • Prof. Steve Scherer
• 17 min
  2. Array comparative genomic hybridization to characterize copy number variation in the human genome

  • Dr. Nigel Carter
• 32 min
  3. CNVs in human genomes

  • Prof. Chris Ponting
• 32 min
  4. Gene copy number variation in human and primate evolution

  • Prof. James Sikela
• 64 min
  5. Genomic disorders: mechanisms for copy number variation and clinical implementation of high-resolution genome analysis

  • Prof. James Lupski
• 44 min
  6. CNVs and clinical diagnosis

  • Dr. Brynn Levy
• 43 min
  7. Quantitative CNV testing in molecular diagnostics

  • Prof. Dimitri J. Stavropoulos
• 33 min
  8. Mendelian CNV mutations

  • Prof. Joris Vermeesch
• 40 min
  9. Copy number variation in neuropsychiatric disorders

  • Dr. Christian Marshall
• 41 min
  10. Copy number variation in association studies of human disease

  • Dr. Steven McCarroll
• 21 min
  11. Ethical considerations in dealing with CNV information

  • Dr. Holly Tabor
• 38 min
  12. The future of CNVs: sequence based resolution and links to human disease 1

  • Prof. Evan Eichler
• 31 min
  13. The future of CNVs: sequence based resolution and links to human disease 2

  • Prof. Evan Eichler
• 31 min
  14. Williams-Beuren syndrome locus: a model of CNV affecting gene dosage and phenotypes

  • Dr. Lucy Osborne
• Archived Lectures *These may not cover the latest advances in the field
• 26 min
  15. Population genetics of structural variation

  • Dr. Don Conrad
• 47 min
  16. Databases for CNV in control and disease populations

  • Dr. Lars Feuk
• 45 min
  17. Copy number variation in mental retardation

  • Dr. Joris Veltman
• 35 min
  18. The case for offering all women amniocentesis and chromosomal microarray analysis

  • Prof. Arthur Beaudet
• 34 min
  19. Structural variants and susceptibility to common human disorders

  • Prof. Xavier Estivill
• 40 min
  20. Indels, CNVs and the spectrum of human genome variation

  • Prof. Samuel Levy
• 36 min
  21. Genome structure and expression

  • Prof. Alexandre Reymond
• 39 min
  22. Quantitative CNV testing in molecular diagnostics

  • Prof. Martin Somerville

Printable Handouts

PDF

Navigable Slide Index

1. Introduction
2. Outline
3. Comparative genome hybridisation
4. Conventional CGH
5. Metaphase spread hybridised in CGH experiment
6. CGH on microarrays
7. 1Mb resolution array
8. Array CGH
9. Array CGH - example
10. Conventional CGH vs. array CGH
11. CNVs are present in normal individuals
12. Assaying CNVs - BAC arrays
13. Genome tilepath array (1)
14. Genome tilepath array (2)
15. Survey of CNVs in human population
16. Survey of CNVs in human population - results
17. Assaying CNVs - long oligo G-W arrays
18. Whole-genome long oligo array
19. Long oligo array vs. BAC array
20. Resolution of the long oligo array
21. Assaying CNVs - fosmid arrays
22. Fosmid arrays
23. BAC vs. fosmid array
24. Redundant fosmid arrays
25. Analysis of chromosome 22 breakpoint (1)
26. Analysis of chromosome 22 breakpoint (2)
27. Assaying CNVs - small insert library arrays
28. Identification of chromosome specific markers
29. BAC vs. fosmid vs. small-insert clones array
30. Assaying CNVs - PCR amplicon arrays
31. PCR amplicon arrays
32. PCR amplicon arrays - example
33. Assaying CNVs - tiling long oligo arrays
34. Resolution of the 42M probe oligo array (1)
35. Resolution of the 42M probe oligo array (2)
36. CNV at 42M oligo-tiling resolution
37. Complexity of known CNVs on chr1
38. Perspectives
39. Summary
40. Acknowledgements

Topics Covered

• Array Comparative Hybridization (array-CGH) and measurement of copy number
• Development of array-CGH technology from convention CGH on metaphase chromosomes
• Initial use for identifying copy number changes in tumors
• Identification of normal copy number variation
• Increasing resolution of array-CGH reveals the scope, complexity and importance of normal copy number variation

Links

Series:

• Copy Number Variation

Categories:

• Genetics & Epigenetics
• Methods

Talk Citation

Carter, N. (2009, August 30). Array comparative genomic hybridization to characterize copy number variation in the human genome [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved August 31, 2024, from https://doi.org/10.69645/UOEI4279.
Export Citation (RIS)

Publication History

• Published on August 30, 2009

Financial Disclosures

• Dr. Nigel Carter has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.