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- Co-ordination of G1 Progression
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37 min
1. START control in yeast- Prof. Curt Wittenberg
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33 min
2. The pRB/E2F pathway- Prof. Jacqueline Lees
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35 min
3. Cell cycle control by the ubiquitin system in mammals- Prof. Michele Pagano
- Chromosome Duplication
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47 min
4. Replication licensing- Prof. Julian Blow
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51 min
5. Initiation of DNA replication- Prof. Bruce Stillman
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48 min
6. Regulation of replication fork progression and stability- Dr. Luis Aragón
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35 min
7. Nucleosome assembly during DNA replication- Dr. Alain Verreault
- Preparing for Mitosis
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27 min
8. Sister chromatid cohesion: simple concept, complex reality- Prof. Douglas Koshland
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50 min
9. Mitotic chromosome condensation- Prof. Andrew Belmont
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33 min
10. Centrosome duplication and separation in animal cells- Prof. Andrew Fry
- Spindle Assembly and Chromosome Segregation
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30 min
11. Bipolar spindle assembly- Dr. Eric Karsenti
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54 min
12. Chromosome biorientation in yeast- Prof. Mike Stark
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43 min
- Mitotic Exit and Cytokinesis
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39 min
14. Cleavage furrow formation and ingression during animal cytokinesis- Dr. Pier Paolo D'Avino
- Checkpoints Governing Cell Cycle Progression
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34 min
15. The DNA damage response- Dr. Vincenzo Costanzo
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54 min
16. The spindle checkpoint- Dr. Kevin Hardwick
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27 min
17. Spindle movement and checkpoint control during mitosis in yeast- Prof. John Cooper
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43 min
18. The G2/M transition- Prof. Dr. René Medema
- The Cell Cycle in Development and Cancer
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62 min
19. Mouse models to investigate cell cycle and cancer- Dr. Philipp Kaldis
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18 min
20. Cell cycle: a complex network of signals regulating cell proliferation- Prof. Antonio Giordano
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40 min
21. Drug discovery and target validation in the p53 pathway- Prof. Sir David Lane
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30 min
22. Role and regulation of Cdk inhibitors in development and cancer- Prof. Martine Roussel
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48 min
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47 min
24. The Myc transcription factor network- Prof. Robert N. Eisenman
- Meiosis: A Specialized Cell Cycle
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58 min
25. Recombination and the formation of chiasmata in meiosis- Prof. Matthew Whitby
- Archived Lectures *These may not cover the latest advances in the field
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24 min
26. Geometric regulation of kinetochore orientation- Prof. Yoshinori Watanabe
Printable Handouts
Navigable Slide Index
- Introduction
- The cell cycle
- Crystal structure of Cdk2 bound to cyclin A
- Periodicity in cyclin expression
- Regulation of Cdks
- Focusing on Cdk2
- Cdk2
- Cdk2-/- knockout mice
- Cdk2-/- knockout mice are viable
- Atrophy in testis of Cdk2-/- mice
- Absence of oocyte development in Cdk2-/- mice
- Expression of cell cycle regulators
- Kinase activity and Cdk/cyclin complexes
- Growth defect in Cdk2-/- MEFs
- Cdk complexes and activity in MEFs
- Cdk2-/- MEF analysis after starvation
- Cdk activity in synchronized MEFs
- Delayed immortalization in Cdk2-/- MEFs
- Summary Cdk2
- Is there another gene that compensates for Cdk2?
- The Retinoblastoma (Rb) protein pathway
- Cdk2-/-Cdk4-/- double knockout mice
- Double mutants die around E15
- Heart defects in Cdk2-/-Cdk4-/- embryos
- In vivo proliferation in Cdk2-/-Cdk4-/- embryos
- Hypophosphorylated Rb
- Impaired proliferation in Cdk2-/-Cdk4-/- MEFs
- S phase entry defect in Cdk2-/-Cdk4-/- MEFs
- Rb phosphorylation in Cdk2-/-Cdk4-/- MEFs
- Rb defect in Cdk2-/-Cdk4-/- MEFs
- Senescence in Cdk2-/-Cdk4-/- MEFs
- HPV-E7
- HPV-E7 mutants
- Effect of Cdk2-/-Cdk4-/- on Cdk1 expression
- Summary Cdk2-/-Cdk4-/-
- What is going on in the S phase?
- The p27 network
- Cdk2-/-p27-/- double knockout mice
- Cdk2-/- and p27-/- mice vs. WT
- Cdk2-/-p27-/- mice
- Ovary tumors in Cdk2-/-p27-/- mice
- Pituitary tumors
- Normal levels of cell death in the thymus
- High levels of S phase and mitosis in thymus
- Cdk activity in the absence of Cdk2 and p27
- Cdk1 binds to p27 and cyclin E
- Proliferation of MEFs
- Silencing of Cdk1 in Cdk2-/- MEFs
- Summary Cdk2-/-p27-/-
- Cdk1/cyclin E complexes can drive S phase
- Cdk1 compensates for Cdk2
- Premature translocation to the nucleus of Cdk1
- DNA damage response in Cdk2-/- knockout mice
- Response to gamma-irradiation in MEFs (1)
- Response to gamma-irradiation in MEFs (2)
- Partial hepatectomy model for in vivo response
- Molecular outcome of gamma-irradiation
- Co-localization of Cdk1 and p21
- Proliferation of MEFs after irradiation
- Cdk2-/- mice are sensitive to gamma-irradiation
- DNA damage: comet assay
- DNA damage: gamma-H2AX staining
- Disturbed kinetics of DNA damage foci
- DNA damage pathway
- DNA damage in the absence of Cdk2
- Acknowledgments
Topics Covered
- Cell cycle regulation during mouse development and adulthood
- Cyclin-dependent kinases (Cdks)
- The genetic pathways for cell cycle regulation
- Cdk1, Cdk2, Cdk4, and Cdk6
- The in vivo functions of Cdk2
- Compensation between different Cdk/cyclin complexes
- Genetic interaction between Cdk2 and Cdk4
- Control of the G1/S phase transition by the Retinoblastoma protein (Rb)
- DNA damage response in the absence of Cdk2
- p27Kip1 inhibits Cdk1 in vivo
- Cdk1/cyclin E complexes promote S phase
- Cdks and tumors
Update Available
The speaker addresses developments since the publication of the original talk. We recommend listening to the associated update as well as the lecture.
- Full lecture Duration: 61:43 min
- Update Interview Duration: 11:32 min
Talk Citation
Kaldis, P. (2020, May 28). Mouse models to investigate cell cycle and cancer [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved January 26, 2021, from https://hstalks.com/bs/1269/.Publication History
Financial Disclosures
- Dr. Philipp Kaldis has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.
