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Printable Handouts
Navigable Slide Index
- Introduction
- mRNA structure
- The cap binding complex
- Arabidopsis ABH1 is the large subunit of the CBC
- Phenotypes of abh1-1 mutant plants (1)
- Phenotypes of abh1-1 mutant plants (2)
- Altered phyllotaxy in abh1-1 mutant plants
- SERRATE mutation effect
- RNA silencing in plants
- Model for RNA silencing by microRNAs (miRNAs)
- Arabidopsis whole genome tiling array (WGA)
- Tiling microarray analysis
- pri-miRs transcripts accumulate in abh1-1 mutants
- Confirmation by qPCR analysis
- Testing abh1-1 effect on levels of mature miRNAs
- abh1-1 effect on mature miRNAs
- abh1-1 effect on 21nucleotide sRNAs
- ABH1 is required for proper mature miRNA levels
- Validating the sequencing data with northern blot
- Schematic of 5'-RACE experiments
- 5'-RACE experiments: results
- Enhanced defects in ABH1 AGO1 double mutants
- ABH1 AGO1 double mutants - fused cotyledons
- ABH1 AGO1 double mutants - a unique defect
- Part A - conclusions
- Utilization of microarrays - part B
- Introduction to part B: RNA degradation
- RNA degradation pathways
- The exosome is required for proper development
- Inducible system for studying exosome function
- Using inducible system in conjunction with WGA
- The exosome regulates specific pri-miRNAs (1)
- The regulated 5' fragments are polyadenylated
- 5' fragments accumulate upon exosome depletion
- Exosome is required for turning over 5' miRNA
- The exosome regulates specific pri-miRNAs (2)
- Exosome depletion doesn't effect mature miRNAs
- Exosome specific transcripts (1)
- Exosome specific transcripts (2)
- A genome wide atlas of exosome substrates (1)
- A genome wide atlas of exosome substrates (2)
- Overall conclusion
- Acknowledgements
Topics Covered
- mRNA structure
- The cap-binding complex is required for proper mRNP assembly
- Phenotypes of abh1-1 mutant plants
- Altered phyllotaxy in abh1-1 mutant plants
- RNA silencing in plants
- Arabidopsis whole genome tiling array
- High-throughput sequencing of the smRNA-component of the Arabidopsis transcriptome
- ABH1 is required for proper mature miRNA levels
- 5'-RACE experiments
- Loss of ABH1 enhances the developmental defects of a hypomorphic AGO1 allele
- RNA degradation
- Exosome function in Arabidopsis
- Tiling arrays suggest the exosome regulates specific fragments of primary miRNA transcripts
- Many of the new transcription units only seen upon exosome-depletion are associated with smRNAs and repetitive elements
- A genome-wide atlas of exosome substrates in Arabidopsis
Talk Citation
Gregory, B. (2009, September 10). Utilizing microarrays to study posttranscriptional regulatory mechanisms of plants [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved December 7, 2024, from https://doi.org/10.69645/IZDF7725.Export Citation (RIS)
Publication History
Financial Disclosures
- Dr. Brian Gregory has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.
Utilizing microarrays to study posttranscriptional regulatory mechanisms of plants
A selection of talks on Cell Biology
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