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1. Introduction
2. Regulation of gene expression
3. Eukaryotic gene expression
4. Translational control of gene expression
5. Genome-wide analysis of gene translation
6. Total RNA vs. polysome-bound RNA
7. Sucrose gradient fractionation of cytosol
8. Cell radioresponse
9. Radiation induced gene expression
10. The effect of radiation through the 2 approaches
11. Radiation modulated genes
12. Transcription vs. translation
13. Hypothesis regarding the effect of radiation
14. Radiation and polysomal mRNA distribution
15. Proteins predicted to increase after irradiation
16. Proteins predicted to decrease after irradiation
17. GOstat
18. Ingenuity pathway analysis (IPA)
19. Radiation induced changes: glioma comparison
20. GOstat: glioma comparison
21. IPA: glioma comparison
22. Radiation induced gene expression: summary
23. Radiation effect vs. tissue of origin
24. Genes affected by 2Gy vs. cell line
25. Genes commonly affected in cells lines
26. Genes common to each tumor type
27. Commonly affected genes: tumor vs. normal
28. Cancer vs. normal cells: biological networks
29. Top ranked network in tumor cells
30. Top ranked network in normal cells
31. Radiation induced translational profiles
32. Summary
33. Acknowledgments
34. References

Topics Covered

• Radiation-induced gene expression has long been considered to play a role in determining cellular radioresponse
• Analyses of single genes and whole genomes indicate that radiation-induced changes at the mRNA level do not correlate with changes in corresponding proteins
• Microarray analysis of polysome-bound RNA indicates that radiation modifies the translational activity of mRNA subpopulations, which correlates with protein products
• Gene translation is considerably more susceptible to radiation-induced changes than is the transcriptome
• Defining the genes whose translation is modified by radiation may provide an approach for identifying critical proteins and pathways that serve as determinants of radiosensitivity

Links

Series:

• Microarrays

Categories:

• Cell Biology
• Methods

Talk Citation

Publication History

• Published on March 30, 2009
• Archived on February 25, 2021

Financial Disclosures

• Prof. Philip Tofilon has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.