• Introduction
• 26 min
  1. From DNA to proteins: the multiple levels of regulation

  • Prof. Panagiotis Tsonis
• Chromatin and DNA
• 44 min
  2. Chromatin architecture and alterations in the control of gene regulation

  • Prof. Jeffrey Hansen
• 43 min
  3. Nucleosome dynamics and remodeling

  • Prof. Jonathan Widom
• 25 min
  4. DNA replication

  • Prof. Smita Patel
• Transcription
• 47 min
  5. Nucleotide dependent functioning of bacterial enhancer binding proteins, activators of sigma54 RNA polymerase

  • Prof. Martin Buck
• 44 min
  6. Mechanisms of transcription: the eukaryotic pre-initiation complex

  • Prof. Michael Hampsey
• RNA (I)
• 38 min
  7. Pre-mRNA splicing

  • Prof. Timothy Nilsen
• 60 min
  8. Self-splicing intron RNAs: ribozymes, parasites and agents of genomic change

  • Prof. Anna Marie Pyle
• 47 min
  9. Polyadenylation of mRNA precursors: mechanism, regulation and connections with other cellular processes

  • Prof. James Manley
• 46 min
  10. mRNA capping

  • Prof. Aaron Shatkin
• RNA (II)
• 40 min
  11. Nonsense-mediated and Staufen1-mediated mRNA decay: related pathways of post-transcriptional control in mammalian cells

  • Prof. Lynne Maquat
• 40 min
  12. The silent revolution: an introduction to gene regulation by microRNAs

  • Dr. Frank Slack
• 50 min
  13. RNA editing: changing the code in plants, animals and parasites

  • Prof. Stephen Hajduk
• Proteins
• 18 min
  14. The principle of translation

  • Prof. Joachim Frank
• 17 min
  15. Translation initiation

  • Prof. Patrick Linder
• 47 min
  16. Elongation of protein synthesis: structural basis of the process of decoding

  • Prof. Marina Rodnina
• 40 min
  17. Elongation of protein synthesis: structural basis of the peptide bond

  • Prof. Marina Rodnina
• 44 min
  18. How do proteins fold and why?

  • Prof. Walter Englander
• 55 min
  19. Protein degradation

  • Prof. Alfred Goldberg
• Understanding Global Gene Expression
• 33 min
  20. Comparing transcriptomes of distant organisms: the comparative ENCODE resource 1

  • Prof. Mark Gerstein
• 22 min
  21. Comparing transcriptomes of distant organisms: models of gene expression 2

  • Prof. Mark Gerstein
• 47 min
  22. Protein networks and analysis of global gene expression

  • Dr. Trey Ideker
• Archived Lectures *These may not cover the latest advances in the field
• 42 min
  23. Chromatin and transcription

  • Prof. Roger Kornberg
• 40 min
  24. Nuclear organization and gene expression

  • Prof. David Spector
• 35 min
  25. Gene and protein microarrays: tools for gene discovery and function

  • Prof. Craig R. Tomlinson

Printable Handouts

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Navigable Slide Index

1. Introduction
2. Gene expression induced by extracellular ligand
3. Nuclear organization
4. Chromosomes arrangement in interphase nucleus
5. Visualizing gene expression in living cells
6. Characterization of the U2OS stable cell line
7. The locus is stably integrated in chr. 1p36
8. 2.5 h post-transfection (-) Dox
9. 2.5 h post-transfection + 2.5 h after Dox addition
10. Evidence that RNA is present at transcription site
11. Changes in chromatin structure after activation (1)
12. Changes in chromatin structure after activation (2)
13. 3-D organization of gene expres. 2 h after initiation
14. Nuclear mRNP dynamics consistent with diffusion
15. mRNP particle tracking
16. Tet-ON-VP16-YFP at the locus after Dox addition
17. Tet-ON-VP16-YFP recruited to the locus over time
18. RNA pol. II large subunit recruited to active locus
19. SF2/ASF recruited to active locus
20. CstF-64 recruited to active locus
21. Tri-methyl. histone H3 as a marker for HP1 binding
22. HP1 alpha/beta/gamma enriched at inactive locus
23. Tri-methyl. histone H3 enriched at inactive locus
24. Suv39h1/G9a-L HMTases localize at inactive locus
25. HP1 alpha clears from locus upon transcription (1)
26. HP1 alpha clears from locus upon transcription (2)
27. HP1 alpha clears from locus upon transcription (3)
28. Histone H3 and the H3.3 variant
29. Histone H3.3 enriched at the active locus (1)
30. Histone H3.3 enriched at the active locus (2)
31. Summary (1)
32. Summary (2)
33. Acknowledgements

Topics Covered

• Current understanding of the organization of the mammalian cell nucleus and how this organization impacts on gene expression
• in-vivo studies of gene expression as a result of the concerted processes of transcription, mRNA processing, messenger ribonucleoprotein particle (mRNP) export and translation
• studies of spatial and temporal aspects of critical cellular processes within the context of the living cell
• development of a cell system that allows the visualization of a transcription site and its mRNA and protein products directly in living cells
• evaluation of spatial and temporal changes in chromatin structure, mRNA synthesis and factor association/disassociation during the transition from a transcriptionally inactive to an active state
• understanding of the in-vivo dynamics of gene expression will provide the basis from which to elucidate spatial and/or temporal alterations that occur in cells associated with various disease states

Links

Series:

• Epigenetics
• From DNA to Proteins

Categories:

• Biochemistry
• Cell Biology
• Genetics & Epigenetics

Talk Citation

Publication History

• Published on October 1, 2007
• Archived on May 31, 2018

Financial Disclosures

• Prof. David Spector has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.