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Printable Handouts
Navigable Slide Index
- Introduction
- Gene expression induced by extracellular ligand
- Nuclear organization
- Chromosomes arrangement in interphase nucleus
- Visualizing gene expression in living cells
- Characterization of the U2OS stable cell line
- The locus is stably integrated in chr. 1p36
- 2.5 h post-transfection (-) Dox
- 2.5 h post-transfection + 2.5 h after Dox addition
- Evidence that RNA is present at transcription site
- Changes in chromatin structure after activation (1)
- Changes in chromatin structure after activation (2)
- 3-D organization of gene expres. 2 h after initiation
- Nuclear mRNP dynamics consistent with diffusion
- mRNP particle tracking
- Tet-ON-VP16-YFP at the locus after Dox addition
- Tet-ON-VP16-YFP recruited to the locus over time
- RNA pol. II large subunit recruited to active locus
- SF2/ASF recruited to active locus
- CstF-64 recruited to active locus
- Tri-methyl. histone H3 as a marker for HP1 binding
- HP1 alpha/beta/gamma enriched at inactive locus
- Tri-methyl. histone H3 enriched at inactive locus
- Suv39h1/G9a-L HMTases localize at inactive locus
- HP1 alpha clears from locus upon transcription (1)
- HP1 alpha clears from locus upon transcription (2)
- HP1 alpha clears from locus upon transcription (3)
- Histone H3 and the H3.3 variant
- Histone H3.3 enriched at the active locus (1)
- Histone H3.3 enriched at the active locus (2)
- Summary (1)
- Summary (2)
- Acknowledgements
Topics Covered
- Current understanding of the organization of the mammalian cell nucleus and how this organization impacts on gene expression
- in-vivo studies of gene expression as a result of the concerted processes of transcription, mRNA processing, messenger ribonucleoprotein particle (mRNP) export and translation
- studies of spatial and temporal aspects of critical cellular processes within the context of the living cell
- development of a cell system that allows the visualization of a transcription site and its mRNA and protein products directly in living cells
- evaluation of spatial and temporal changes in chromatin structure, mRNA synthesis and factor association/disassociation during the transition from a transcriptionally inactive to an active state
- understanding of the in-vivo dynamics of gene expression will provide the basis from which to elucidate spatial and/or temporal alterations that occur in cells associated with various disease states
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Talk Citation
Spector, D. (2007, October 1). Nuclear organization and gene expression [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved December 6, 2024, from https://doi.org/10.69645/HBCB7891.Export Citation (RIS)
Publication History
Financial Disclosures
- Prof. David Spector has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.