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1. Introduction
2. The cellular organelles
3. The secretory pathway in plants
4. Understanding the endoplasmic reticulum
5. From gene to protein: introduction
6. Proteins fold up in an aqueous environment
7. Incorrect protein folding
8. Aggregates can be formed
9. Protein folding is assisted by chaperones
10. Chaperone action also costs energy
11. Difference between prokaryotes and eukaryotes (1)
12. Difference between prokaryotes and eukaryotes (2)
13. Transcription and translation in eukaryotes
14. Targeting proteins to the secretory pathway (1)
15. The endoplasmic reticulum is a special case
16. Protein synthesis starts in the cytosol
17. Protein synthesis on the rough ER
18. The ER is the entry point to the secretory pathway
19. Targeting proteins to the secretory pathway (2)
20. Targeting proteins to the secretory pathway (3)
21. Targeting proteins to the secretory pathway (4)
22. Entry into the ER lumen
23. The ER contains a special chaperone: BiP
24. BiP plays a crucial role in protein translocation (1)
25. BiP plays a crucial role in protein translocation (2)
26. BiP plays a crucial role in protein translocation (3)
27. BiP plays a crucial role in protein translocation (4)
28. Classification of membrane proteins - type 1
29. Classification of membrane proteins - type 2
30. Some proteins span the membrane many times
31. A range of topologies is possible
32. Coding region of a type I membrane protein
33. Coding region of another type I membrane protein
34. Type I membrane protein translocation (1)
35. Type I membrane protein translocation (2)
36. Coding region of a type II membrane protein
37. Type II proteins are translocated post-translation
38. Export from the ER to the Golgi
39. Receptor-mediated transport
40. What is a model?
41. Export from the ER to the Golgi - another look
42. Clearly it cannot be that simple
43. Necessity of retrograde transport from the Golgi
44. How to introduce fluorescent markers into the ER
45. Leaf infiltration with Agrobacterium: a compromise
46. 1-3 days later: sampling for microscopy
47. A tiny portion of the leaf epidermis
48. Focus on the surface of the cells
49. The ER in plants forms a very dynamic network
50. Electron microscopy of immunogold labelled ER
51. Golgi bodies move over the tubular ER network
52. ER export sites and Golgi bodies
53. The high plasticity of the plant ER network
54. Recombinant molecules in ER membranes
55. Further reading

Topics Covered

• The secretory pathway in plants
• The endoplasmic reticulum is the entry point into the secretory pathway
• Protein entry in the ER
• Protein synthesis on the rough endoplasmic reticulum
• Protein folding
• Protein export from the ER
• Export from the ER to the Golgi
• Retrograde transport from the Golgi
• Techniques to study the plant ER

Links

Series:

• The Endoplasmic Reticulum

Categories:

• Cell Biology
• Plant & Animal Sciences

Talk Citation

Publication History

• Published on October 1, 2007
• Updated on October 1, 2014

Financial Disclosures

• Dr. Jurgen Denecke has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.