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1. Introduction
2. Cellular Ca signaling
3. Two phases of Ca signaling
4. Two sources of Ca in signaling
5. Ca release and entry
6. Could IP3 be the signal for Ca entry?
7. The regulation of the cellular Ca pool
8. Regulation of Ca release by carbachol and atropine
9. Refilling Ca pool
10. Capacitative calcium entry - the model
11. SERCA inhibitors
12. The effect of a SERCA inhibitor: thapsigargin
13. Summary: Ca exchange
14. The effect of TG on Ca stores and Ca exchange
15. ICRAC: Ca release-activated Ca current
16. Varying ionic selectivity of CCE channels
17. Capacitative Ca entry (CCE)
18. The pharmacology of CCE
19. CCE: key questions
20. Signaling for capacitative Ca entry channel
21. DT40 B-lymphocytes - IP3R-KO
22. Partial purification of CIF
23. ICRAC activation in cell-lines
24. ICRAC in RBL cells activated by IP3
25. CIF: conclusions
26. RNAi KO of STIM1 in HEK293 cells
27. Structure of STIM1
28. STIM1 and SOC activation
29. Imaging EYFP-STIM1
30. EYFP-STIM1 with CCh and Atrp
31. HEK293 cells co-transfection: YFP-STIM1 / M5-R
32. EYFP-STIM1 and TG
33. TIRF imaging: EYFP-STIM1
34. EF hand of STIM1
35. EYFP-STIM1-D76N, D78N
36. The effect of STIM1 mutant on Ca signaling
37. The TRP superfamily
38. Structural features of mammalian TRPCs
39. The structure of a functional channel
40. Regulation of TRPC channels by store depletion
41. HEK293 cells
42. DT40 B lymphocytes
43. DT40 B cells
44. TRPC3 in wild type DT40 Cells
45. Low vs. high expression of TRPC3 in DT40 B cells
46. Signaling pathways for HTRPC3-mediated Ca entry
47. Functional store-operated channels
48. Incomplete and non-functional channels
49. Acknowledgements
50. Addendum

Topics Covered

• The concept of capacitative or store-operated calcium entry
• Icrac
• Pharmacology of store-operated entry
• Theories of activation mechanisms
• CIF
• Stim1
• TRP channels

Links

Series:

• Calcium Signaling I

Categories:

• Biochemistry
• Cell Biology

Talk Citation

Publication History

• Published on October 1, 2007
• Archived on August 13, 2020

Financial Disclosures

• Dr. Jim Putney has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.