We noted you are experiencing viewing problems
-
Check with your IT department that JWPlatform, JWPlayer and Amazon AWS & CloudFront are not being blocked by your network. The relevant domains are *.jwplatform.com, *.jwpsrv.com, *.jwpcdn.com, jwpltx.com, jwpsrv.a.ssl.fastly.net, *.amazonaws.com and *.cloudfront.net. The relevant ports are 80 and 443.
-
Check the following talk links to see which ones work correctly:
Auto Mode
HTTP Progressive Download Send us your results from the above test links at access@hstalks.com and we will contact you with further advice on troubleshooting your viewing problems. -
No luck yet? More tips for troubleshooting viewing issues
-
Contact HST Support access@hstalks.com
-
Please review our troubleshooting guide for tips and advice on resolving your viewing problems.
-
For additional help, please don't hesitate to contact HST support access@hstalks.com
We hope you have enjoyed this limited-length demo
This is a limited length demo talk; you may
login or
review methods of
obtaining more access.
Printable Handouts
Navigable Slide Index
- Introduction
- Talk outline
- Nanoscale electrochemical sensors
- In situ detection of small molecules
- Bacterial cell-cell communication
- The incredible pyocyanin molecule
- Electrochemistry 101
- Single bacterium redox molecule detection
- Issues with miniaturized reference electrodes
- Microfabricated devices
- Device fabrication in depth
- Sacrificial layer etch
- A closer look at the sensor
- The complete setup
- Characterization of the sensor
- Electrochemical techniques
- Measuring pyocyanin: current/potential
- Measuring pyocyanin: current/concentration
- Detecting pyocyanin in P. aeruginosa cultures
- Pyocyanin in P. aeruginosa cultures: results
- Pyocyanin precursors
- Pyocyanin precursors: proposed mechanism
- Microfluidic device with integrated sensors
- Future electrochemical studies
- Early infection detection electrochemical sensors
- Antibiotic-resistant bacteria
- ESKAPE pathogens
- Pseudomonas aeruginosa (Info from CDC)
- Electrochemical scans of biological fluids
- Scans of pyocyanin in various biological fluids
- Limit of detection (pyocyanin)
- Pyocyanin specificity
- Preventing biofilm formation
- Resistance & minimum inhibitory concentration
- Pyocyanin production is linked with cell viability
- Making sensors more sensitive
- Early detection of infections
- Amino acid addition, biofilm up-regulation
- Up-regulating pyocyanin production
- Up-regulating biofilm formation using amino acids
- Varying the initial bacterial concentration
- Isolation and cultivation of new micro-organisms
- Uncultivable species
- Isolating new micro-organisms: diffusion chambers
- High throughput automated isolation & cultivation
- Isolation chamber fabrication
- Nano-constriction device fabrication
- Bacterial growth through constrictions
- Bacterial motility in constrictions
- Sorting 2 different bacteria species
- Mixture of GFP P. aeruginosa & m-cherry E. coli
- Isolating individual cells from the same species
- Separating marine species
- Anaerobic species experiments
- Future work
- Selective isolation of species
- Spatially-controlled biofilm formation
- Localized biofilms
- Conclusions and future work
- Goluch group & acknowledgements
- Northeastern University
- Interdisciplinary science & engineering complex
- Contact and funding information
Topics Covered
- Abilities of bacteria to organize and adapt in dynamic situations
- Electrochemical sensors for bacterial analysis and real-time infection detection
- Micro/nano-fluidic devices to trap and isolate individual bacterial species
Links
Series:
Categories:
Therapeutic Areas:
Talk Citation
Goluch, E. (2015, May 4). Nanosensors for determining the presence of bacteria [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved September 1, 2024, from https://doi.org/10.69645/ECJW6022.Export Citation (RIS)
Publication History
Financial Disclosures
- Prof. Edgar Goluch, Grant/Research Support (Principal Investigator): U.S. National Science Foundation Stock Shareholder (Self-managed): QSM Diagnostics, Inc.
Other Talks in the Series: Nanomedicine
Transcript
Please wait while the transcript is being prepared...
0:00
Hello, I'm Dr. Edgar
Goluch from the Department
of Chemical Engineering
at Northeastern University
in Boston, Massachusetts.
And today, I'll be telling you
about nanosensors for determining
the presence of bacteria.
0:14
My research group works on
a number of topics related
to bacterial cells and
we're primarily focused
on developing sensing and fluidic
systems to detect and examine both
individual cells and biofilms.
Today, I'll be focusing on two types
of devices that we're developing.
First, we'll talk about
electrochemical sensors
and how they're made and how we're
applying them for early infection
detection and then
secondly, we'll talk
about some microfluidic
devices that you
can use to isolate and cultivate
microorganisms, as well as
detect the presence of infections.
0:53
So to begin with, let's look at some
nanoscale electrochemical sensors.
0:60
The reason why we're interested
in these types of sensors
is to look at small molecules
that bacteria and all cells
produce and excrete
into their environment.
So you might be familiar with
neurons, for example, releasing
dopamine, serotonin, and
other neurotransmitters
into their local environment
for the other cells
to respond to and collect.
1:26
Bacteria do something very similar.
They communicate with each other
both through the same species
and to other species to coordinate
their activities, to determine when
to form biofilms, when
to release toxins,
or when to move to new locations
when they're being attacked
or when food sources run out.
All of this is done
with small molecule
communications which can be detected
using electrochemical sensors.