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Printable Handouts
Navigable Slide Index
- Introduction
- Stem cells
- Bristlecone pine
- Control of stem cell division
- microRNA and stem cells in model organisms
- microRNA function in stem cells
- Characteristics of microRNAs
- Mutating Dicer and Drosha in Drosophila GSCs
- Dcr-1 mutants
- Germ-line stem cells location
- GSC maintenance and self renewal
- Notch pathway activation induces extra niche cells
- GSC maintenance: niche to GSC signaling
- Drosophila Dicer is required for GSC maintenance
- Dicer and Mad are required for GSC maintenance
- Analysis of microRNAs expression in GSC
- miR-7, miR-8 and bantam are expressed in GSCs
- Which microRNA is required in GSC?
- Bantam is required for GSC self-renewal
- Signaling pathways in GSC self-renewal: summary
- microRNA control of stem cell division
- Dicer and Mad in Drosophila larval/pupal clones
- Drosophila oogenesis
- Drosophila oogenesis in Dcr-1 mutants
- Dcr1-mutant stem cells stop dividing!
- Dcr1-mutant stem cells halt in G1/S transition
- Is Dacapo a direct target of microRNAs?
- Analysis of Dacapo regulation
- Dacapo promoter is not responsive to Dicer
- Do microRNAs directly regulate Dap 3'UTR?
- Dap 3'UTR is responsive to miRNAs in GSCs
- Dap 3'UTR is responsive to miR-7 and -309
- microRNA in Drosophila GSC: summary (1)
- microRNA in Drosophila GSC: summary (2)
- microRNA function in hESCs
- Dicer and Drosha knock-down
- Lentiviral construct
- Confirm knockdown by QPCR
- mRNA profiling in KD cells
- Drosha and Dicer KD hESC(H1)- super stem cells
- Dicer KD cells have higher level of Oct4 protein
- Division rate of Dicer and Drosha KD hESCs(H1)
- Cell cycle status of the mutant hESCs(H1)
- p21 protein is elevated in Dicer KD cells
- microRNA profiling in H7, H1 and HSF6 cells
- hESCs express a unique set of microRNAs
- Downregulation of microRNAs upon differentiation
- Differentiating vs. undifferentiated ES cells
- Rescue of Dicer KD phenotypes
- Summary
- Acknowledgements
Topics Covered
- microRNAs
- Stem cells
- Stem cell cell cycle
- Stem cell checkpoints
- p21
- hESCs
- Stem cell self-renewing division
- Notch
- Niche
Talk Citation
Ruohola-Baker, H. (2008, September 29). microRNA function in stem cells [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved December 7, 2024, from https://doi.org/10.69645/JPAH9964.Export Citation (RIS)
Publication History
Financial Disclosures
- Prof. Hannele Ruohola-Baker has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.