Printable Handouts

PDF

Navigable Slide Index

1. Introduction
2. Stem cells
3. Bristlecone pine
4. Control of stem cell division
5. microRNA and stem cells in model organisms
6. microRNA function in stem cells
7. Characteristics of microRNAs
8. Mutating Dicer and Drosha in Drosophila GSCs
9. Dcr-1 mutants
10. Germ-line stem cells location
11. GSC maintenance and self renewal
12. Notch pathway activation induces extra niche cells
13. GSC maintenance: niche to GSC signaling
14. Drosophila Dicer is required for GSC maintenance
15. Dicer and Mad are required for GSC maintenance
16. Analysis of microRNAs expression in GSC
17. miR-7, miR-8 and bantam are expressed in GSCs
18. Which microRNA is required in GSC?
19. Bantam is required for GSC self-renewal
20. Signaling pathways in GSC self-renewal: summary
21. microRNA control of stem cell division
22. Dicer and Mad in Drosophila larval/pupal clones
23. Drosophila oogenesis
24. Drosophila oogenesis in Dcr-1 mutants
25. Dcr1-mutant stem cells stop dividing!
26. Dcr1-mutant stem cells halt in G1/S transition
27. Is Dacapo a direct target of microRNAs?
28. Analysis of Dacapo regulation
29. Dacapo promoter is not responsive to Dicer
30. Do microRNAs directly regulate Dap 3'UTR?
31. Dap 3'UTR is responsive to miRNAs in GSCs
32. Dap 3'UTR is responsive to miR-7 and -309
33. microRNA in Drosophila GSC: summary (1)
34. microRNA in Drosophila GSC: summary (2)
35. microRNA function in hESCs
36. Dicer and Drosha knock-down
37. Lentiviral construct
38. Confirm knockdown by QPCR
39. mRNA profiling in KD cells
40. Drosha and Dicer KD hESC(H1)- super stem cells
41. Dicer KD cells have higher level of Oct4 protein
42. Division rate of Dicer and Drosha KD hESCs(H1)
43. Cell cycle status of the mutant hESCs(H1)
44. p21 protein is elevated in Dicer KD cells
45. microRNA profiling in H7, H1 and HSF6 cells
46. hESCs express a unique set of microRNAs
47. Downregulation of microRNAs upon differentiation
48. Differentiating vs. undifferentiated ES cells
49. Rescue of Dicer KD phenotypes
50. Summary
51. Acknowledgements

Topics Covered

• microRNAs
• Stem cells
• Stem cell cell cycle
• Stem cell checkpoints
• p21
• hESCs
• Stem cell self-renewing division
• Notch
• Niche

Links

Series:

• The Legacy of Drosophila Genetics

Categories:

• Cell Biology

Talk Citation

Publication History

• Published on September 29, 2008
• Archived on February 25, 2021

Financial Disclosures

• Prof. Hannele Ruohola-Baker has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.