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1. Introduction
2. Protein sorting in the eukaryotic cell
3. Intracellular transport of proteins
4. Intracellular distribution of radioautographic grains
5. The endoplasmic reticulum
6. Newly synthesized proteins sequestered in ER
7. Precursor/processed forms of secretory proteins
8. A mechanism for protein translocation
9. The signal hypothesis: Gunter Blobel
10. Topogenic signal directs protein translocation
11. Signal sequence structure
12. How is the signal sequence recognized?
13. Ribosome/ nascent chain/ SRP complexes
14. SRP/SRP receptor-directed targeting of ribosomes
15. Schematic overview of protein translocation
16. Mechanism of protein translocation in the ER
17. Sec61p: the ER protein conducting channel
18. The "translocon" composition
19. Sampling of the ER membrane bilayer
20. Protein conducting channel identification in the ER
21. Membrane proteins integration model: part I
22. Membrane proteins integration model: part II
23. Translational regulation on the ER
24. mRNA translation rates are governed by PERK
25. mRNA segregation in the cell
26. Summary

Topics Covered

• Protein sorting in the eukaryotic cell
• The discovery of the secretory pathway
• Overview discussion of the structure/function of the endoplasmic reticulum
• Experimental protocols and landmark experiments leading to the discovery of protein translocation
• The signal hypothesis
• Signal sequence structure
• Signal sequence recognition
• SRP receptor and targeting to the ER
• Mechanism of protein translocation and the protein translocation channel
• Translational regulation on the ER
• The unfolded protein response
• mRNA partitioning in the cell
• Are there alternative pathways for compartmentalizing protein synthesis?

Links

Series:

• The Endoplasmic Reticulum

Categories:

• Cell Biology

Talk Citation

Nicchitta, C. (2007, October 1). Endoplasmic reticulum, protein synthesis and translocation machinery [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved April 15, 2025, from https://doi.org/10.69645/PHPW8297.
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Publication History

• Published on October 1, 2007

Financial Disclosures

• Dr. Christopher Nicchitta has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.