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- Bioorganic Chemistry and the Origins of Chemical Biology
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56 min
1. Biomimetic catalysis- Prof. Ronald Breslow
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50 min
2. Bioorganic studies of vision- Prof. Koji Nakanishi
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48 min
3. Perspectives on biological catalysis- Prof. Stephen Benkovic
- Nucleic Acids and Drug/Nucleic Acid Interactions
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41 min
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27 min
5. Site-specifically modified ribosomal RNAs- Prof. Christine Chow
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26 min
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48 min
7. Drug-DNA interactions of the mitomycins- Prof. Maria Tomasz
- Protein Function and Cellular Signaling
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41 min
8. Chemical synthesis of proteins- Prof. Lei Liu
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55 min
9. Visualizing and manipulating phosphoinositide and phospholipid signaling- Prof. Glenn Prestwich
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86 min
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59 min
11. Polydactyl zinc finger proteins: software and hardware for genomes- Prof. Carlos Barbas
- Peptides, Small-Molecules and Chemical Diversity
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41 min
12. Chemical synthesis of peptides and peptide libraries- Prof. Victor Hruby
- Proteomics: The Study of Biomolecules and Interactions
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46 min
13. Quantitative proteomics- Prof. Ruedi Aebersold
- Archived Lectures *These may not cover the latest advances in the field
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51 min
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57 min
15. Solid phase peptide synthesis- Prof. Stephen Kent
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39 min
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32 min
17. Tagged library for chemical genetics- Dr. Young-Tae Chang
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30 min
18. Covalently constrained alpha-helices- Dr. Paramjit Arora
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34 min
19. SICLOPPS: genetic system for production of backbone cyclized peptides- Dr. Sergey Savinov
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35 min
Printable Handouts
Navigable Slide Index
- Introduction
- Challenges in the post-genomic era
- Functional assignments through perturbation
- Small-molecule antagonism of protein complexes
- Extraordinary solutions for a nontrivial problem
- Systematic search for protein modulators (1)
- Bifunctional and mini-inteins
- Chemistry of intein splicing
- Cyclizative ligation: the concept
- SICLOPPs
- Peptides as biological probes
- Cyclic vs. linear
- Intracellular production
- Recombinant cyclic peptides
- Implementation of the concept
- Affinity chromatography
- SDS-PAGE analysis
- Cyclic peptide detection
- Intracellular production of bioactive peptide
- Library construction
- Scope and limitations
- Epitope 'grafting' for templated splicing
- Streptavidin-binding peptide
- Binding strength
- Linking function to survival
- Systematic search for protein modulators (2)
- Conditional transcription system (1)
- Conditional transcription system (2)
- Systematic search for protein modulators (3)
- Ribonucleotide reductase: dissociative inhibition
- Searching for dissociative inhibitors
- Dissociative inhibitors?
- Protein-protein dissociation assay (1)
- Protein-protein dissociation assay (2)
- Peptide display ELISA
- Conclusions
- Acknowledgements
Topics Covered
- SICLOPPS: method for production of genetically encoded backbone cyclized peptides
- Cyclic peptides as probes of biological function
- Splicing of a circularly rearranged split-intein
- Production and isolation of backbone cyclic peptides
- Generation of combinatorial libraries
- Selection of protein-protein inhibitors using a bacterial two-hybrid assay
- Biochemical characterization of dissociative inhibitors
Talk Citation
Savinov, S. (2008, August 14). SICLOPPS: genetic system for production of backbone cyclized peptides [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved April 2, 2025, from https://doi.org/10.69645/VBQH7562.Export Citation (RIS)
Publication History
Financial Disclosures
- Dr. Sergey Savinov has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.
SICLOPPS: genetic system for production of backbone cyclized peptides
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