• Bioorganic Chemistry and the Origins of Chemical Biology
• 56 min
  1. Biomimetic catalysis

  • Prof. Ronald Breslow
• 50 min
  2. Bioorganic studies of vision

  • Prof. Koji Nakanishi
• 48 min
  3. Perspectives on biological catalysis

  • Prof. Stephen Benkovic
• Nucleic Acids and Drug/Nucleic Acid Interactions
• 41 min
  4. The importance of nucleotide size in the chemistry and biology of DNA

  • Prof. Eric Kool
• 27 min
  5. Site-specifically modified ribosomal RNAs

  • Prof. Christine Chow
• 26 min
  6. Creation of tools for the investigation of oxidative damage to nucleic acids

  • Dr. Mel Bedi
• 48 min
  7. Drug-DNA interactions of the mitomycins

  • Prof. Maria Tomasz
• Protein Function and Cellular Signaling
• 55 min
  8. Visualizing and manipulating phosphoinositide and phospholipid signaling

  • Prof. Glenn Prestwich
• 86 min
  9. Phosphoserine/threonine binding domains: molecular integrators of protein kinase signaling in cell cycle control and cancer

  • Dr. Michael Yaffe
• 59 min
  10. Polydactyl zinc finger proteins: software and hardware for genomes

  • Prof. Carlos Barbas
• Peptides, Small-Molecules and Chemical Diversity
• 41 min
  11. Chemical synthesis of peptides and peptide libraries

  • Prof. Victor Hruby
• Proteomics: The Study of Biomolecules and Interactions
• 46 min
  12. Quantitative proteomics

  • Prof. Ruedi Aebersold
• Archived Lectures *These may not cover the latest advances in the field
• 51 min
  13. Chemical protein synthesis: total synthesis of proteins for biological research

  • Prof. Stephen Kent
• 57 min
  14. Solid phase peptide synthesis

  • Prof. Stephen Kent
• 39 min
  15. Mapping small molecule binding sites with phage display technology

  • Dr. Lee Makowski
• 32 min
  16. Tagged library for chemical genetics

  • Dr. Young-Tae Chang
• 30 min
  17. Covalently constrained alpha-helices

  • Dr. Paramjit Arora
• 34 min
  18. SICLOPPS: genetic system for production of backbone cyclized peptides

  • Dr. Sergey Savinov
• 35 min
  19. Probing protein-protein interactions with peptide libraries and arrays

  • Dr. Zhou Songyang

Printable Handouts

PDF

Navigable Slide Index

1. Introduction
2. Challenges in the post-genomic era
3. Functional assignments through perturbation
4. Small-molecule antagonism of protein complexes
5. Extraordinary solutions for a nontrivial problem
6. Systematic search for protein modulators (1)
7. Bifunctional and mini-inteins
8. Chemistry of intein splicing
9. Cyclizative ligation: the concept
10. SICLOPPs
11. Peptides as biological probes
12. Cyclic vs. linear
13. Intracellular production
14. Recombinant cyclic peptides
15. Implementation of the concept
16. Affinity chromatography
17. SDS-PAGE analysis
18. Cyclic peptide detection
19. Intracellular production of bioactive peptide
20. Library construction
21. Scope and limitations
22. Epitope 'grafting' for templated splicing
23. Streptavidin-binding peptide
24. Binding strength
25. Linking function to survival
26. Systematic search for protein modulators (2)
27. Conditional transcription system (1)
28. Conditional transcription system (2)
29. Systematic search for protein modulators (3)
30. Ribonucleotide reductase: dissociative inhibition
31. Searching for dissociative inhibitors
32. Dissociative inhibitors?
33. Protein-protein dissociation assay (1)
34. Protein-protein dissociation assay (2)
35. Peptide display ELISA
36. Conclusions
37. Acknowledgements

Topics Covered

• SICLOPPS: method for production of genetically encoded backbone cyclized peptides
• Cyclic peptides as probes of biological function
• Splicing of a circularly rearranged split-intein
• Production and isolation of backbone cyclic peptides
• Generation of combinatorial libraries
• Selection of protein-protein inhibitors using a bacterial two-hybrid assay
• Biochemical characterization of dissociative inhibitors

Links

Series:

• Chemical Biology

Categories:

• Biochemistry
• Methods

Talk Citation

Publication History

• Published on August 14, 2008
• Archived on February 25, 2021

Financial Disclosures

• Dr. Sergey Savinov has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.