• Architecture of the ribosome RNA-protein machine assembly
• 49 min
  1. High resolution structure of ribosomal subunits RNA/RNA and protein/RNA interactions

  • Prof. Nenad Ban
• 38 min
  2. Assembly of the 30S ribosomal subunit in vitro and in cells

  • Prof. James Williamson
• Decoding and peptide bond formation
• 46 min
  3. How aminoacyl-tRNA synthetases translate the genetic code

  • Dr. Stephen Cusack
• 42 min
  4. The structural basis for how the large ribosomal subunit catalyses peptide bond formation

  • Prof. Thomas Steitz
• 47 min
  5. Elongation of protein synthesis: structural basis of the process of decoding

  • Prof. Marina Rodnina
• Initiation of protein synthesis
• 49 min
  6. The structure-based mechanism of translation initiation in bacteria and eukaryotes

  • Prof. Jeffrey Kieft
• 48 min
  7. Factor independent initiation of protein synthesis by IRES RNAs

  • Prof. Jeffrey Kieft
• Elongation and termination of protein synthesis
• 41 min
  8. Translocation: movement of tRNA and mRNA through the ribosome

  • Prof. Harry Noller
• 31 min
  9. Recoding: getting more out of the message by shifting reading frame and redefining codon meaning

  • Prof. John Atkins
  • Prof. Raymond Gesteland
• Co-translational protein secretion
• 75 min
  10. Mechanism of translocon function: current insights and models

  • Prof. Arnold Driessen
• How antibiotics target the ribosome
• 29 min
  11. Antibiotics that target the 50S ribosome subunit: resistance and other considerations

  • Dr. Joyce Sutcliffe
• 35 min
  12. Antibiotic inhibition of ribosome function

  • Dr. Daniel Wilson
• 45 min
  13. Structure-based drug design targeting infectious disease

  • Dr. Erin Duffy
• Archived Lectures *These may not cover the latest advances in the field
• 26 min
  14. Structural insights into aminoacyl-tRNA delivery by EF-Tu and translocation by EF-G

  • Prof. Joachim Frank
• 21 min
  15. Introduction to the ribosome

  • Prof. Anders Liljas
• 20 min
  16. The structure of the intact ribosome and ribosomal subunit interactions

  • Dr. Jamie H. Doudna Cate
• 42 min
  17. Structural insights into decoding of mRNA by the ribosome

  • Prof. Venki Ramakrishnan
• 63 min
  18. Chemistry of peptide bond formation

  • Prof. Rachel Green
• 49 min
  19. The interaction of antibiotics with the large ribosomal subunit from Haloarcula marismortui

  • Prof. Peter Moore

Printable Handouts

PDF

Navigable Slide Index

1. Introduction
2. The central dogma
3. tRNA is the central player in translation
4. The probability of errors in protein synthesis
5. Selectivity and transition state
6. The genetic code
7. Decoding - selection of the correct tRNA
8. The bacterial ribosome
9. Antibiotics change the fidelity of the ribosome
10. Fidelity mutations far from the anticodon
11. Selection of aa-tRNA by the ribosome (1)
12. Problems with the tRNA selection scheme
13. Selection of aa-tRNA by the ribosome (2)
14. The atomic structure of the 30S subunit
15. First clue of how decoding might work
16. Testing the decoding model
17. Induced fit on tRNA binding
18. Minor groove recognition
19. Near-cognate Leu(II) ASL in the 30S
20. Structures with cognate and near-cognate ASL
21. Binding of Leu(II) tRNA
22. Tautomers and base pairs
23. An unusual tautomer at 2nd position?
24. 30S closing movement
25. Ribosome mutations that affect accuracy
26. Energetics of transition to closed form
27. Domain closure leads to tRNA selection
28. tRNA orientations
29. TC loop / tRNA summary
30. An integrated view of decoding
31. Base pairing and fidelity
32. Synchrotrons
33. Acknowledgements

Topics Covered

• tRNA: the central player in translation
• Selectivity and transition state
• Decoding: selection of the correct tRNA
• The bacterial ribosome
• Antibiotics change the fidelity of the ribosome
• Selection of aa-tRNA by the ribosome
• The atomic structure of the 30S subunit
• Testing the decoding model
• Minor groove recognition of base pairing geometry
• Tautomers and base pairs
• Streptomycin
• Base pairing and fidelity

Links

Series:

• The Mechanisms of Ribosome Function

Categories:

• Biochemistry
• Cell Biology

Talk Citation

Publication History

• Published on May 15, 2008
• Archived on August 13, 2020

Financial Disclosures

• Prof. Venki Ramakrishnan has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.