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- Architecture of the ribosome RNA-protein machine assembly
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2. Assembly of the 30S ribosomal subunit in vitro and in cells
- Prof. James Williamson
- Decoding and peptide bond formation
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3. How aminoacyl-tRNA synthetases translate the genetic code
- Dr. Stephen Cusack
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5. Elongation of protein synthesis: structural basis of the process of decoding
- Prof. Marina Rodnina
- Initiation of protein synthesis
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7. Factor independent initiation of protein synthesis by IRES RNAs
- Prof. Jeffrey Kieft
- Elongation and termination of protein synthesis
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8. Translocation: movement of tRNA and mRNA through the ribosome
- Prof. Harry Noller
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9. Recoding: getting more out of the message by shifting reading frame and redefining codon meaning
- Prof. John Atkins
- Prof. Raymond Gesteland
- Co-translational protein secretion
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10. Mechanism of translocon function: current insights and models
- Prof. Arnold Driessen
- How antibiotics target the ribosome
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12. Antibiotic inhibition of ribosome function
- Dr. Daniel Wilson
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13. Structure-based drug design targeting infectious disease
- Dr. Erin Duffy
- Archived Lectures *These may not cover the latest advances in the field
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15. Introduction to the ribosome
- Prof. Anders Liljas
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16. The structure of the intact ribosome and ribosomal subunit interactions
- Dr. Jamie H. Doudna Cate
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17. Structural insights into decoding of mRNA by the ribosome
- Prof. Venki Ramakrishnan
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18. Chemistry of peptide bond formation
- Prof. Rachel Green
Printable Handouts
Navigable Slide Index
- Introduction
- The central dogma
- tRNA is the central player in translation
- The probability of errors in protein synthesis
- Selectivity and transition state
- The genetic code
- Decoding - selection of the correct tRNA
- The bacterial ribosome
- Antibiotics change the fidelity of the ribosome
- Fidelity mutations far from the anticodon
- Selection of aa-tRNA by the ribosome (1)
- Problems with the tRNA selection scheme
- Selection of aa-tRNA by the ribosome (2)
- The atomic structure of the 30S subunit
- First clue of how decoding might work
- Testing the decoding model
- Induced fit on tRNA binding
- Minor groove recognition
- Near-cognate Leu(II) ASL in the 30S
- Structures with cognate and near-cognate ASL
- Binding of Leu(II) tRNA
- Tautomers and base pairs
- An unusual tautomer at 2nd position?
- 30S closing movement
- Ribosome mutations that affect accuracy
- Energetics of transition to closed form
- Domain closure leads to tRNA selection
- tRNA orientations
- TC loop / tRNA summary
- An integrated view of decoding
- Base pairing and fidelity
- Synchrotrons
- Acknowledgements
Topics Covered
- tRNA: the central player in translation
- Selectivity and transition state
- Decoding: selection of the correct tRNA
- The bacterial ribosome
- Antibiotics change the fidelity of the ribosome
- Selection of aa-tRNA by the ribosome
- The atomic structure of the 30S subunit
- Testing the decoding model
- Minor groove recognition of base pairing geometry
- Tautomers and base pairs
- Streptomycin
- Base pairing and fidelity
Talk Citation
Ramakrishnan, V. (2008, May 15). Structural insights into decoding of mRNA by the ribosome [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved March 20, 2025, from https://doi.org/10.69645/NWAT9316.Export Citation (RIS)
Publication History
Financial Disclosures
- Prof. Venki Ramakrishnan has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.