• Architecture of the ribosome RNA-protein machine assembly
• 49 min
  1. High resolution structure of ribosomal subunits RNA/RNA and protein/RNA interactions

  • Prof. Nenad Ban
• 38 min
  2. Assembly of the 30S ribosomal subunit in vitro and in cells

  • Prof. James Williamson
• Decoding and peptide bond formation
• 46 min
  3. How aminoacyl-tRNA synthetases translate the genetic code

  • Dr. Stephen Cusack
• 42 min
  4. The structural basis for how the large ribosomal subunit catalyses peptide bond formation

  • Prof. Thomas Steitz
• 47 min
  5. Elongation of protein synthesis: structural basis of the process of decoding

  • Prof. Marina Rodnina
• Initiation of protein synthesis
• 49 min
  6. The structure-based mechanism of translation initiation in bacteria and eukaryotes

  • Prof. Jeffrey Kieft
• 48 min
  7. Factor independent initiation of protein synthesis by IRES RNAs

  • Prof. Jeffrey Kieft
• Elongation and termination of protein synthesis
• 41 min
  8. Translocation: movement of tRNA and mRNA through the ribosome

  • Prof. Harry Noller
• 31 min
  9. Recoding: getting more out of the message by shifting reading frame and redefining codon meaning

  • Prof. John Atkins
  • Prof. Raymond Gesteland
• Co-translational protein secretion
• 75 min
  10. Mechanism of translocon function: current insights and models

  • Prof. Arnold Driessen
• How antibiotics target the ribosome
• 29 min
  11. Antibiotics that target the 50S ribosome subunit: resistance and other considerations

  • Dr. Joyce Sutcliffe
• 35 min
  12. Antibiotic inhibition of ribosome function

  • Dr. Daniel Wilson
• 45 min
  13. Structure-based drug design targeting infectious disease

  • Dr. Erin Duffy
• Archived Lectures *These may not cover the latest advances in the field
• 26 min
  14. Structural insights into aminoacyl-tRNA delivery by EF-Tu and translocation by EF-G

  • Prof. Joachim Frank
• 21 min
  15. Introduction to the ribosome

  • Prof. Anders Liljas
• 20 min
  16. The structure of the intact ribosome and ribosomal subunit interactions

  • Dr. Jamie H. Doudna Cate
• 42 min
  17. Structural insights into decoding of mRNA by the ribosome

  • Prof. Venki Ramakrishnan
• 63 min
  18. Chemistry of peptide bond formation

  • Prof. Rachel Green
• 49 min
  19. The interaction of antibiotics with the large ribosomal subunit from Haloarcula marismortui

  • Prof. Peter Moore

Printable Handouts

PDF

Navigable Slide Index

1. Introduction
2. Talk outline - introduction
3. Protein synthesis by the ribosome
4. Ribosome size
5. Talk outline - composition and landmarks
6. Ribosomal components
7. tRNA and mRNA binding sites
8. View of 70S ribosome from 30S solvent side (1)
9. View of 70S ribosome from A-site tRNA side
10. Talk outline - subunit interface
11. View of 70S ribosome from 30S solvent side (2)
12. Ribosomal subunit interface has an rRNA core
13. RNA minor groove packing at interface: bridge B3
14. Solvation at the subunit interface: bridge B6
15. Mg2+ ion dependence of subunit association
16. Mg2+ ion conservation in ribosomals subunit
17. One specific Mg2+ ion binding site (1)
18. One specific Mg2+ ion binding site (2)
19. Solvation at the subunit interface near bridge B2c
20. Flexible bridge at the edge of interface: bridge B4
21. Hydrophobic packing in bridge B4
22. Flexibility in the core of the interface: h44
23. Flexibility at the core of the interface: bridge B2a
24. Talk outline - conformational changes
25. mRNA and tRNA translocation reaction
26. Ribosome dynamics during translocation: (1)
27. Ratching of the 30S subunit around bridge B3
28. Ribosome dynamics during translocation: (2)
29. Swiveling of the 30S subunit head
30. Swiveling follows tRNA trajectory motion
31. Bridge B1 changes in different ribosomal states
32. Ribosome dynamics during translocation (3)
33. View of tRNA groove from A-tRNA side
34. Unlocking the ribosome in translocation (1)
35. Unlocking the ribosome in translocation (2)
36. Unlocking the ribosome in translocation (3)
37. Ribosome dynamics during translocation (4)
38. 30S head swiveling due to overwinding of h28
39. Dynamics of h28 in the human 80S ribosome
40. Ribosome dynamics during translocation (5)
41. L1 arm (H76) moves in and out of interface region
42. The mechanism of L1 arm movement
43. Ribosome dynamics during translocation (6)
44. L11 arm movement concentrated in RNA helix H42
45. Ribosome dynamics during translocation (7)
46. L7/L12 stalk is highly dynamic
47. Talk outline - concluding thoughts
48. Summary - protein synthesis by the ribosome

Topics Covered

• Introduction to the architecture of the bacterial ribosome
• Ribosome composition and landmarks
• Ribosomal subunit interface
• Conformational changes in the ribosome

Links

Series:

• The Mechanisms of Ribosome Function

Categories:

• Biochemistry
• Cell Biology

Talk Citation

Publication History

• Published on May 15, 2008
• Archived on August 13, 2020

Financial Disclosures

• Dr. Jamie H. Doudna Cate has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.