• Architecture of the ribosome RNA-protein machine assembly
• 49 min
  1. High resolution structure of ribosomal subunits RNA/RNA and protein/RNA interactions

  • Prof. Nenad Ban
• 38 min
  2. Assembly of the 30S ribosomal subunit in vitro and in cells

  • Prof. James Williamson
• Decoding and peptide bond formation
• 46 min
  3. How aminoacyl-tRNA synthetases translate the genetic code

  • Dr. Stephen Cusack
• 42 min
  4. The structural basis for how the large ribosomal subunit catalyses peptide bond formation

  • Prof. Thomas Steitz
• 47 min
  5. Elongation of protein synthesis: structural basis of the process of decoding

  • Prof. Marina Rodnina
• Initiation of protein synthesis
• 49 min
  6. The structure-based mechanism of translation initiation in bacteria and eukaryotes

  • Prof. Jeffrey Kieft
• 48 min
  7. Factor independent initiation of protein synthesis by IRES RNAs

  • Prof. Jeffrey Kieft
• Elongation and termination of protein synthesis
• 41 min
  8. Translocation: movement of tRNA and mRNA through the ribosome

  • Prof. Harry Noller
• 31 min
  9. Recoding: getting more out of the message by shifting reading frame and redefining codon meaning

  • Prof. John Atkins
  • Prof. Raymond Gesteland
• Co-translational protein secretion
• 75 min
  10. Mechanism of translocon function: current insights and models

  • Prof. Arnold Driessen
• How antibiotics target the ribosome
• 29 min
  11. Antibiotics that target the 50S ribosome subunit: resistance and other considerations

  • Dr. Joyce Sutcliffe
• 35 min
  12. Antibiotic inhibition of ribosome function

  • Dr. Daniel Wilson
• 45 min
  13. Structure-based drug design targeting infectious disease

  • Dr. Erin Duffy
• Archived Lectures *These may not cover the latest advances in the field
• 26 min
  14. Structural insights into aminoacyl-tRNA delivery by EF-Tu and translocation by EF-G

  • Prof. Joachim Frank
• 21 min
  15. Introduction to the ribosome

  • Prof. Anders Liljas
• 20 min
  16. The structure of the intact ribosome and ribosomal subunit interactions

  • Dr. Jamie H. Doudna Cate
• 42 min
  17. Structural insights into decoding of mRNA by the ribosome

  • Prof. Venki Ramakrishnan
• 63 min
  18. Chemistry of peptide bond formation

  • Prof. Rachel Green
• 49 min
  19. The interaction of antibiotics with the large ribosomal subunit from Haloarcula marismortui

  • Prof. Peter Moore

Printable Handouts

PDF

Navigable Slide Index

1. Introduction
2. How is a polypeptide synthesized? (1)
3. How is a polypeptide synthesized? (2)
4. 1940
5. DNA
6. The genetic code
7. tRNA (1)
8. Crick's adaptor hypothesis, 1956
9. sRNA - tRNA
10. tRNA (2)
11. tRNA: the substrate of translation
12. The charging system - 20 tRNA synthetases
13. RNA and ribosomes (1)
14. Ribosomes seen as black dots by EM
15. RNA and ribosomes (2)
16. 2D gel of the ribosomal proteins from E. coli
17. Composition of ribosomes
18. Relationship between ribosomal proteins
19. 1964 - function is structure
20. Electron microscopy of ribosomes
21. Localization of ribosomal proteins
22. Some ribosomal proteins have unusual structure
23. 1978 - E. coli rRNA
24. tRNA sites
25. 2000 - crystal structures of ribosomes
26. Ribosomal sites (1)
27. Phases of translation
28. Bacterial translation factors
29. Ribosomal sites (2)
30. EF-Tu
31. The GTP binding consensus elements of EF-G
32. The EF-Tu cycle
33. Interactions with the ternary complex seen on EM
34. Dissociation of EF-Tu
35. Is the ribosome a ribozyme?
36. EF-G translocates pp-tRNA from A- to P-site
37. EF-G bound to the ribosome
38. When and how does L12 interact?
39. L12 3-D structure
40. L7/L12 dimer by NMR
41. L12 seen by NMR in 70S ribosomes
42. The elongating ribosome binding of aa-tRNA
43. Translocation by EF-G
44. EF-G interaction surface on L12
45. RF2 has almost the shape of a tRNA
46. Conformational changes seen from 3-D structures
47. Ribosome recycling RRF mimics tRNA
48. Neither RF2 nor RRF bind as tRNA

Topics Covered

• The understanding of heredity and translation of proteins based on the genetic information
• Intensive structural studies of ribosomes, tRNAs and translation factors
• A historical overview
• Main components of translation

Links

Series:

• The Mechanisms of Ribosome Function

Categories:

• Biochemistry
• Cell Biology

Talk Citation

Publication History

• Published on May 15, 2008
• Archived on August 13, 2020

Financial Disclosures

• Prof. Anders Liljas has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.