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1. Introduction
2. Lecture outline
3. The blood-brain-barrier
4. Cellular architecture of the BBB
5. Pericytes
6. Molecular characteristics of the BBB
7. Reference in vitro models of the BBB
8. Cultured brain microvascular endothelial cells
9. Phenotypic markers of brain endothelial cell
10. BBB in vitro models
11. Example of a co-culture system
12. In vitro permeability assay
13. Trans-endothelial electrical resistance (TEER)
14. In vitro and in vivo drug permeability
15. Drug permeability of various in vitro models
16. Immortalization of rat brain endothelial cells
17. Primary BMECs vs. immortalized cell lines
18. A new model of human BBB
19. Immortalization of human brain endothelial cells
20. hCMEC/D3: a non-transformed phenotype (1)
21. hCMEC/D3: a non-transformed phenotype (2)
22. hCMEC/D3: expression of junction proteins (1)
23. hCMEC/D3: expression of junction proteins (2)
24. Permeability characteristics of hCMEC/D3 cells
25. Efflux pupms: ABC transporters
26. hCMEC/D3: functionality of ABC transporters
27. The hCMEC/D3 cell line
28. The hCMEC/D3 model under flow
29. hCMEC/D3 in a DIV-BBB device
30. Hyperosmolar opening of BBB in DIV models
31. Radioactive sucrose permeability in DIV models
32. hCMEC/D3 in a DIV-BBB device: conclusions
33. Applications of in vitro models of the BBB
34. BBB: physiological and pathophysiological roles
35. In vitro BBB models and research axes
36. Leukocyte infiltration across the BBB
37. Response to cell adhesion and transmigration
38. ICAM-1and PECAM-1 signaling
39. Tight junctions, receptors and transporters
40. Analysis of TJ proteomes in hCMEC/D3 cells
41. Multiple reaction monitoring (MRM)
42. Analysis by quantitative mass spectrometry
43. Optimizing drug delivery to the brain
44. BBB in vitro models: conclusions, perspectives
45. Acknowledgments

Topics Covered

• Blood-brain barrier
• In vitro models of blood-brain barrier
• Blood-brain barrier permeability
• Brain endothelium and tight junctions
• Transporters at the blood-brain barrier

Links

Series:

• The Blood-Brain Barrier

Categories:

• Methods
• Neuroscience
• Pharmaceutical Sciences

Therapeutic Areas:

• Neurology

Talk Citation

Publication History

• Published on March 1, 2008
• Archived on October 28, 2018

Financial Disclosures

• Prof. Pierre-Olivier Couraud has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.