• Introduction and General Properties of Plasmids
• 31 min
  1. Introduction to plasmid biology

  • Prof. Emeritus Don Clewell
• 39 min
  2. General properties and applications of plasmids

  • Prof. Emeritus Don Clewell
• Replication
• 47 min
  3. Plasmids: copy number control by antisense RNAs

  • Prof. Gerhart Wagner
• 30 min
  4. Plasmid rolling-circle (RC) replication

  • Prof. Saleem Khan
• 45 min
  5. Replication of linear plasmids in bacteria

  • Prof. George Chaconas
• Maintenance and Partitioning
• 32 min
  6. Plasmid segregation and stability in bacteria

  • Prof. Barbara Funnell
• 33 min
  7. Plasmid stabilization by cell killing

  • Prof. Keith Weaver
• 30 min
  8. Dimer catastrophes and plasmid resolution

  • Dr. David Summers
• Conjugation and Mobilization
• 41 min
  9. The diversity of bacterial conjugation and mobilization systems

  • Prof. Fernando de la Cruz
• 36 min
  10. The pCF10-encoded pheromone response: cell-cell signaling, complexity and evolution

  • Prof. Gary Dunny
• 27 min
  11. DNA processing in conjugative transfer

  • Dr. Joel Schildbach
• 43 min
  12. Agrobacterium tumefaciens

  • Dr. Stephen Winans
• Genetic Traits Frequently Associated with Plasmids
• 37 min
  13. Insertion sequences and DNA transposition

  • Prof. William Reznikoff
• 48 min
  14. Plasmids, integrons and the spread of antibiotic resistance

  • Prof. Hatch Stokes
• 30 min
  15. Virulence plasmids

  • Prof. Julian Rood
• Eukaryotic Plasmids
• 30 min
  16. The yeast plasmid: a highly persistent selfish DNA element

  • Prof. Makkuni Jayaram
• Population Biology and Evolution
• 35 min
  17. Evolution of plasmids and their role in bacterial diversity and adaptability

  • Prof. Christopher Thomas
• Archived Lectures *These may not cover the latest advances in the field
• 85 min
  18. Bacterial extrachromosomal elements: role in chemical detoxification and potential cancer therapy

  • Prof. Ananda Chakrabarty
• 34 min
  19. Plasmid segregation and stability in bacteria

  • Prof. Barbara Funnell
• 39 min
  20. Replication of linear plasmids in bacteria

  • Prof. George Chaconas

Printable Handouts

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Navigable Slide Index

1. Introduction
2. E. faecalis can adapt to many different situations
3. Adaptation can be short-term or longer term
4. Sex-pheromone plasmids and conjugation
5. A genetic map of the pCF10 plasmid
6. Main points of the talk
7. Original 1-way signaling model is overly simple
8. Pheromone synthesis and control circuits (1)
9. Pheromone synthesis
10. The PrgY/TraB family tree
11. Model for PrgY function
12. Part I -summary: action from the membrane out
13. Early regulatory events in pheromone induction
14. PrgX function in PrgQ transcription control
15. Structure of PrgX/cCF10 vs. PrgX
16. Structure of PrgX/iCF10 - C-terminus
17. Integration of structures into working models
18. The remaining issues with the looping model
19. Main points
20. Plasmid pCF10
21. In vivo plasmid transfer
22. Induction of pCF10 transfer by plasma
23. Pheromone synthesis and control circuits (2)
24. PrgB expression increases virulence
25. Evolution and "kluging"
26. Conclusion
27. Acknowledgements
28. References (1)
29. References (2)

Topics Covered

• Overview of the evolutionary and medical significance of plasmids in E faecalis
• Overview of cell-cell signaling and plasmid transfer
• The sex pheromone system
• Pheromone production and control plasmid pCF10 as a model for pheromone-controlled plasmid transfer
• Mechanism of pheromone induction
• Multiple roles for pheromone induction
• Maintenance of complexity in evolution

Links

Series:

• Biology and Significance of Plasmids

Categories:

• Methods
• Microbiology

Therapeutic Areas:

• Infectious Diseases

Talk Citation

Publication History

• Published on January 28, 2008

Financial Disclosures

• Prof. Gary Dunny has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.