Printable Handouts

PDF

Navigable Slide Index

1. Introduction
2. Outline
3. Transfer of DNA between bacterial cells
4. Basic plasmid platforms
5. Tra vs. Mob: R388 and RSF1010
6. The mechanism of conjugation
7. The key components in conjugation
8. TrwB - a coupling protein
9. Related DNA transport processes
10. The mating channel
11. Liquid vs. surface maters
12. The relaxosome
13. Shoot and pump conjugation model
14. The complex between TrwC and its substrate DNA
15. Catalytic center mutagenesis
16. TrwC relaxase protein family
17. Conserved relaxase motifs in six relaxase families
18. 3D relaxase structures
19. Diversity and classification of conjugation systems
20. The IncW family
21. IncW backbone
22. Segregational incompatibility
23. Methods for plasmid classification
24. Well known plasmid Inc groups in Enterobacteria
25. Conserved relaxase motifs in six relaxase families
26. BLAST searches against prototype relaxases
27. MOB-P relaxase family
28. The MOB-F family
29. MOB-H relaxase family
30. MOB-C family
31. Classification by relaxases vs. T4CP and T4SS
32. Plasmid MOB families
33. MOB families distribution and relaxase signatures
34. A search on the NCBI plasmid database
35. Progression in plasmid sequencing
36. Plasmid dstribution in bacterial host phyla
37. Plasmid distribution according to size
38. Conjugative vs. mobilizable plasmids
39. Relaxase families in 1,010 plasmids
40. MOBs distribution according to plasmid size
41. Relaxases distribution in main bacterial phyla
42. Relaxases dstribution in phylum Proteobacteria
43. NCBI plasmid database analysis: conclusions
44. Acknowledgements

Topics Covered

• Transfer of DNA between bacterial cells
• Basic plasmid platforms
• The mechanism and key components of conjugation
• The mating channel
• The complex between TrwC and its substrate DNA
• The relaxosome
• The diversity of relaxases
• The IncW family
• Methods for plasmid classification
• Plasmid MOB families
• The NCBI plasmid database
• Plasmid sequencing

Links

Series:

• Biology and Significance of Plasmids

Categories:

• Genetics & Epigenetics
• Methods
• Microbiology

Talk Citation

de la Cruz, F. (2008, January 28). The diversity of bacterial conjugation and mobilization systems [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved December 22, 2024, from https://doi.org/10.69645/AUCI8961.
Export Citation (RIS)

Publication History

• Published on January 28, 2008

Financial Disclosures

• Prof. Fernando de la Cruz has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.