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1. Introduction
2. Plasmid partition and chromosome dynamics
3. Plasmid stability systems
4. Why do plasmids need partition systems?
5. Plasmid localization in the cell cycle
6. Plasmid partition cassettes
7. Classes of partition systems
8. Type 1: walker ATPases
9. Plasmid partition sites (1)
10. Plasmid partition sites (2)
11. Repressor and corepressors
12. The diversity of plasmids with type 1 par systems
13. Type 2: actin ATPases
14. Plasmids with multiple par systems
15. Mechanistic questions
16. The action of ParA and ParB proteins: ParB
17. The P1 partition complex
18. Visualization of partition complexes
19. ParB structure and function
20. The structure of a partition complex
21. ParBs of Type 1 par systems
22. Small ParBs are RHH2 dimers
23. Omega RHH2 binds DNA
24. The action of ParA and ParB proteins: ParA
25. Plasmid localization requires ParA
26. Measurements of localization
27. Walker-type partition ATPases
28. ParA binds and hydrolyzes ATP
29. ParA is a transcriptional repressor
30. ParA has site-specific DNA binding activity
31. Mutations in ParA reveal ATP interactions
32. The ATP-ADP switch
33. How does ParA mediate plasmid localization?
34. ParM and actin share properties
35. ParM filaments drive plasmids apart
36. ParM polymerization drives R1 partition
37. Do walker-type ATPases polymerize?
38. Partition ATPase dynamics: observations
39. Model: par complex dynamics
40. Remaining questions

Topics Covered

• Mechanisms of plasmid segregation and partition reactions
• Types of plasmid partition systems in bacteria
• ParA partition ATPases
• Structural biology of partition-site binding proteins
• Chromosome dynamics

Links

Series:

• Biology and Significance of Plasmids

Categories:

• Microbiology

Therapeutic Areas:

• Infectious Diseases

Talk Citation

Publication History

• Published on January 28, 2008

Financial Disclosures

• Prof. Barbara Funnell has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.