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1. Introduction
2. Rolling-circle replicating (RCR) plasmids
3. Characteristicts of RCR plasmids
4. Functional organization of RCR plasmids
5. A model for plasmid rolling-circle replication
6. Replication in plasmids carrying two pT181 dsos
7. DNA sequence and structure of the pT181 origin
8. Initiator proteins of RCR plasmids (1)
9. Initiator proteins of RCR plasmids (2)
10. DNA nicking-closing activity of RepC
11. Domain structure of RepC initiator - pT181 plasmid
12. Conservation of the active tyrosine residue
13. DNA binding domains of pT181 family initiators
14. Replication origin of the pT181 family plasmids
15. Rep-DNA specificity
16. Role of individual monomers of the Rep protein
17. Purification of RepC heterodimers
18. SDS-PAGE analysis of purified RepC heterodimers
19. Division of labor between the two monomers
20. PcrA helicase
21. Role of the PcrA helicase in RC replication
22. Conversion of ss to ds DNA
23. General features of single-strand origins (SSOs)
24. Types of single strand origins of replication
25. SsoA origins of RC plasmids
26. Synthesis of primer RNAs from plasmid ssoA
27. Binding of staphylococal RNAP to pLS1/pE194
28. Binding of RNA polymerase to the ssoU
29. ssoA sequences of pLS1 and pE194 plasmids
30. pUB110/pMV158 ssoU
31. Model of lagging-strand synthesis from ssoA
32. Lagging strand replication - conclusions
33. Important unresolved issues

Topics Covered

• Rolling circle replicating (RCR) plasmids
• Functional organization of rolling-circle replicating plasmids
• Model for plasmid rolling-circle replication
• Initiation and termination of replication in plasmids carrying two pT181 dsos
• DNA sequence and structure of the pT181 origin
• Initiator proteins of RCR plasmids
• Comparison of the replication origins of the plasmids of pT181 family
• Rep-DNA specificity
• Role of individual monomers of the Rep protein
• Purification of RepC heterodimers
• PcrA helicase
• Role of PcrA helicase in plasmid RC replication
• Conversion of ss to ds DNA
• Single strand origins of replication
• Lagging-strand replication

Links

Series:

• Biology and Significance of Plasmids

Categories:

• Genetics & Epigenetics
• Methods
• Microbiology

Therapeutic Areas:

• Infectious Diseases

Talk Citation

Publication History

• Published on January 28, 2008

Financial Disclosures

• Prof. Saleem Khan has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.