• Introduction and General Properties of Plasmids
• 31 min
  1. Introduction to plasmid biology

  • Prof. Emeritus Don Clewell
• 39 min
  2. General properties and applications of plasmids

  • Prof. Emeritus Don Clewell
• Replication
• 47 min
  3. Plasmids: copy number control by antisense RNAs

  • Prof. Gerhart Wagner
• 30 min
  4. Plasmid rolling-circle (RC) replication

  • Prof. Saleem Khan
• 45 min
  5. Replication of linear plasmids in bacteria

  • Prof. George Chaconas
• Maintenance and Partitioning
• 32 min
  6. Plasmid segregation and stability in bacteria

  • Prof. Barbara Funnell
• 33 min
  7. Plasmid stabilization by cell killing

  • Prof. Keith Weaver
• 30 min
  8. Dimer catastrophes and plasmid resolution

  • Dr. David Summers
• Conjugation and Mobilization
• 41 min
  9. The diversity of bacterial conjugation and mobilization systems

  • Prof. Fernando de la Cruz
• 36 min
  10. The pCF10-encoded pheromone response: cell-cell signaling, complexity and evolution

  • Prof. Gary Dunny
• 27 min
  11. DNA processing in conjugative transfer

  • Dr. Joel Schildbach
• 43 min
  12. Agrobacterium tumefaciens

  • Dr. Stephen Winans
• Genetic Traits Frequently Associated with Plasmids
• 37 min
  13. Insertion sequences and DNA transposition

  • Prof. William Reznikoff
• 48 min
  14. Plasmids, integrons and the spread of antibiotic resistance

  • Prof. Hatch Stokes
• 30 min
  15. Virulence plasmids

  • Prof. Julian Rood
• Eukaryotic Plasmids
• 30 min
  16. The yeast plasmid: a highly persistent selfish DNA element

  • Prof. Makkuni Jayaram
• Population Biology and Evolution
• 35 min
  17. Evolution of plasmids and their role in bacterial diversity and adaptability

  • Prof. Christopher Thomas
• Archived Lectures *These may not cover the latest advances in the field
• 85 min
  18. Bacterial extrachromosomal elements: role in chemical detoxification and potential cancer therapy

  • Prof. Ananda Chakrabarty
• 34 min
  19. Plasmid segregation and stability in bacteria

  • Prof. Barbara Funnell
• 39 min
  20. Replication of linear plasmids in bacteria

  • Prof. George Chaconas

Printable Handouts

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Navigable Slide Index

1. Introduction
2. Physical detection and isolation of plasmid DNA
3. Buoyant density of the F plasmid
4. Topology and ethidium bromide
5. Binding of ethidium bromide to DNA
6. CsCl-ethidium bromide gradients
7. Topology and alkaline denaturation
8. Covalently closed circles renature
9. Plasmid extraction with alkaline lysis
10. ColE1 relaxation complex
11. Electron microscopy of relaxation complex
12. Agarose gel electrophoresis (AGE)
13. Topoisomerases
14. Topoisomers seen on AGE
15. E. coli/ColE1 DNA replication
16. Restriction enzymes
17. Example of restriction enzyme cleavage
18. AGE showing restriction patterns of plasmids
19. AGE patterns reflecting transposon insertions
20. Topoisomers and restriction fragments in AGE
21. Cleavage at BamHI site
22. Ligation of BamHI ends
23. Modification of BamHI site
24. SmaI cleavage
25. Different restriction endonucleases
26. Recombinant DNA technology
27. Cloning DNA fragments with plasmid vector
28. Transformation of plasmid into E. coli
29. Cloning, expression and screening
30. Cloning vectors for E. coli
31. pAM434 with multiple cloning site
32. Shuttle vectors
33. pAM401 shuttle plasmid
34. Sequencing the cloned DNA
35. Map of pAD1
36. Importance of plasmids

Topics Covered

• Properties of plasmids that facilitate isolation
• Basic analyses of plasmids
• Restriction enzymes and their use as analytical tools
• The important role of plasmids in recombinant DNA technology and genome analyses

Links

Series:

• Biology and Significance of Plasmids

Categories:

• Methods
• Microbiology

Therapeutic Areas:

• Infectious Diseases

Talk Citation

Publication History

• Published on January 28, 2008
• Reviewed on April 16, 2023

Financial Disclosures

• Prof. Emeritus Don Clewell has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.