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1. Intro slide
2. The biological issue: differential mRNA turnover
3. Decay rates of mammalian mRNA
4. mRNA decay as a control in gene expression
5. mRNA decay role in limiting viruses
6. mRNAs protective modifications
7. General pathways of mRNA turnover
8. Specialized pathways of mRNA turnover
9. Targeting of nucleases to specific mRNA
10. Regulation of endonuclease cleavage
11. Individual steps as regulators of decay rate
12. Stability elements as a regulators of decay rates
13. Proteins as a regulators of mRNA degradation
14. Co-regulation of proteins of related functions
15. miRNA as a regulator of mRNA turnover
16. mRNA stability: more regulators
17. Examples of mRNA stability regulation
18. Summary of stability elements
19. Nucleases of mRNA turnover
20. mRNA deadenylases
21. Ccr4/Pop2/NOT complex
22. Pan2/Pan3 complex and PARN
23. Substrate preferences of different deadenylases
24. Implication (1)
25. Implication (2)
26. 3' to 5' exonucleolytic decay
27. Exosome functions
28. Exosome organization
29. An archeal exosome
30. Rrp6 association with the exosome
31. Co-factors of exosome in mRNA degradation
32. Exosome recruitment to the substrate
33. Decapping of the final product
34. DcpS decapping enzyme
35. 5' to 3' exonucleolytic decay
36. Decapping as a control for mRNA decay
37. Dcp1p/Dcp2p decapping enzyme
38. Assembly of mRNA decapping complex
39. Translation and decapping as a distinct states
40. P-bodies and their content (1)
41. P-bodies and their content (2)
42. P-bodies as sites of decapping
43. mRNA cycle
44. Mechanism for translation repression
45. Stress granules
46. Biochemical states of cytoplasmic mRNA
47. P-bodies as translation repressor
48. Regulators of the translation status
49. miRNAs target mRNAs to P-bodies
50. The mRNA decay network
51. Sites of quality control in mRNA turnover
52. Quality control in the nucleus
53. Quality control in the cytoplasm (nonstop decay)
54. The C-terminal domain of ski7p
55. Molecular mimicry?
56. Nonstop decay
57. Premature polyadenylation
58. Nonsense-mediated decay
59. Factors of nonsense-mediated decay
60. Affects of nonsense-mediated decay on turnover
61. Upf1p in the decay of "normal" RNA
62. Aberrant and normal RNA: model in yeast
63. Aberrant and normal RNA: model in mammals
64. Present knowledge
65. References (1)
66. References (2)
67. References (3)

Topics Covered

• Control of mRNA degradation
• Modulation in response to physiological cues
• Mechanisms of mRNA turnover
• Critical nucleases
• Sequence-specific binding of regulators
• Rates of turnover
• mRNA decay and viral infections
• Role in recognizing aberrant mRNAs

Links

Series:

• Eukaryotic Gene Regulation

Categories:

• Cell Biology
• Genetics & Epigenetics

Talk Citation

Parker, R. (2007, October 1). The mechanisms and control of mRNA turnover in eukaryotic cells [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved November 21, 2024, from https://doi.org/10.69645/ZKFD7065.
Export Citation (RIS)

Publication History

• Published on October 1, 2007
• Archived on April 13, 2022

Financial Disclosures

• Prof. Roy Parker has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.