• RNAi-Pathway Components and Mechanisms
• 39 min
  1. Activation of gene expression by double-stranded RNAs

  • Prof. Long-Cheng Li
• 31 min
  2. MicroRNA biogenesis

  • Dr. Narry Kim
• 35 min
  3. Dicer and R2D2

  • Dr. Young Sik Lee
• 26 min
  4. Mammalian microRNA assembly and function

  • Prof. Zissimos Mourelatos
• 33 min
  5. Mechanisms of RNAi: a molecular view or Argonaute: the secret life of Slicer

  • Prof. Leemor Joshua-Tor
• Roles for RNAi in Development and Anti-Viral Defense
• 38 min
  6. Functional roles of RNAi in C. elegans

  • Dr. Eric Miska
• 41 min
  7. RNAi and heterochromatin in plants and fission yeast

  • Prof. Robert Martienssen
• 29 min
  8. RNA interference functions and mechanisms in animals

  • Prof. Richard Carthew
• 40 min
  9. Specific functional roles in mammals

  • Dr. Michael McManus
• RNAi Design Rules for Applications in Humans
• 38 min
  10. siRNA on and off-target

  • Prof. Anastasia Khvorova
• 43 min
  11. Cell signalling pathways and RNAi

  • Mr. Michael O'Grady
• 33 min
  12. RNAi: key technological aspects

  • Dr. Dmitry Samarsky
  • Dr. Ciaran Faherty
• 28 min
  13. RNAi specificity: how big of an issue is it?

  • Dr. Peter Welch
• Applications of RNAi in the Treatment of Human Diseases
• 18 min
  14. siRNA therapeutics

  • Prof. John Rossi
  • Mr. Daniel J. Rossi
• 48 min
  15. RNAi for neurological diseases

  • Prof. Beverly L. Davidson
• 31 min
  16. RNA interference and cancer: a revolution?

  • Dr. Annick Harel-Bellan
• Archived Lectures *These may not cover the latest advances in the field
• 51 min
  17. RNAi and viral heart infections

  • Dr. Jens Kurreck
• 26 min
  18. RNA interference for neurodegenerative diseases

  • Prof. Beverly L. Davidson
• 42 min
  19. Activation of gene expression by double-stranded RNA

  • Prof. David Corey
• 43 min
  20. New designs for siRNAs as Dicer substrate

  • Dr. Mark Behlke
• 27 min
  21. RNAi in the model system Drosophila

  • Prof. Richard Carthew
• 29 min
  22. Small interfering RNA induced transcriptional gene silencing in human cells

  • Dr. Kevin Morris
• 37 min
  23. Therapeutic delivery of RNAi

  • Dr. Patrick Lu
• 44 min
  24. Mechanistic aspects and therapeutic applications of RNAi

  • Prof. John Rossi

Printable Handouts

PDF

Navigable Slide Index

1. Introduction
2. Polyglutamine (polyQ) repeat diseases
3. Repeat expansion diseases (1)
4. Repeat expansion diseases (2)
5. Suppressing dominant disease genes
6. How to accomplish reduction in expression?
7. Reduction in expression by RNA interference
8. A cartoon of the RNAi pathway
9. Human spinocerebellar ataxia type 1 (SCA1)
10. Effects of shSCA1 on mutant human ataxin-1 (1)
11. Effects of shSCA1 on mutant human ataxin-1 (2)
12. Testing shAtaxin1 in vivo: dendritic atrophy
13. Testing shAtaxin1 in vivo: hSCA1 transgenics
14. Testing shAtaxin1 in vivo: ataxia
15. How to accomplish RNAi in target brain cells
16. AAVs - deliver genetic material to the brain (1)
17. AAVs - deliver genetic material to the brain (2)
18. Testing shAtaxin1 in vivo: delivery issues
19. shSCA1 and shLacZ transcripts in cerebellum
20. shSCA1: impact on behavior
21. Advantage of half-transduced lobules
22. Photomicrographs from half-transduced lobules
23. shSCA1 normalizes molecular layer widths
24. shSCA1 reduces ataxin-1 aggregates
25. Summary (1)
26. Huntington's disease: a brief history
27. Huntington's disease (HD)
28. Mutant huntingtin expression downstream effects
29. Designing shRNAs for huntingtin
30. shHD2.1 reduces HD-N171-82Q mRNA
31. shHD2.1 reduces HD-N171-82Q Protein
32. Testing shHD in vivo
33. shRNA delivery to mouse brain using AAV1
34. shHD2.1 reduces transgene expression in vivo
35. shHD2.1 reduces Htt-immunoreactive inclusions
36. shHD2.1 improves rotarod performance in mice
37. Gait abnormalities in HD mice
38. shHD2.1 improves stride length in mice
39. Summary (2)
40. Acknowledgements

Topics Covered

• Brain diseases and molecular therapies
• RNA interference
• Mice models of brain disease
• Viral vectors

Links

Series:

• RNA Interference

Categories:

• Cell Biology
• Genetics & Epigenetics
• Neuroscience
• Pharmaceutical Sciences

Therapeutic Areas:

• Neurology

Talk Citation

Publication History

• Published on October 1, 2007
• Archived on June 22, 2016

Financial Disclosures

• Prof. Beverly L. Davidson has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.