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1. Introduction
2. Polyglutamine (polyQ) repeat diseases
3. Repeat expansion diseases (1)
4. Repeat expansion diseases (2)
5. Suppressing dominant disease genes
6. How to accomplish reduction in expression?
7. Reduction in expression by RNA interference
8. A cartoon of the RNAi pathway
9. Human spinocerebellar ataxia type 1 (SCA1)
10. Effects of shSCA1 on mutant human ataxin-1 (1)
11. Effects of shSCA1 on mutant human ataxin-1 (2)
12. Testing shAtaxin1 in vivo: dendritic atrophy
13. Testing shAtaxin1 in vivo: hSCA1 transgenics
14. Testing shAtaxin1 in vivo: ataxia
15. How to accomplish RNAi in target brain cells
16. AAVs - deliver genetic material to the brain (1)
17. AAVs - deliver genetic material to the brain (2)
18. Testing shAtaxin1 in vivo: delivery issues
19. shSCA1 and shLacZ transcripts in cerebellum
20. shSCA1: impact on behavior
21. Advantage of half-transduced lobules
22. Photomicrographs from half-transduced lobules
23. shSCA1 normalizes molecular layer widths
24. shSCA1 reduces ataxin-1 aggregates
25. Summary (1)
26. Huntington's disease: a brief history
27. Huntington's disease (HD)
28. Mutant huntingtin expression downstream effects
29. Designing shRNAs for huntingtin
30. shHD2.1 reduces HD-N171-82Q mRNA
31. shHD2.1 reduces HD-N171-82Q Protein
32. Testing shHD in vivo
33. shRNA delivery to mouse brain using AAV1
34. shHD2.1 reduces transgene expression in vivo
35. shHD2.1 reduces Htt-immunoreactive inclusions
36. shHD2.1 improves rotarod performance in mice
37. Gait abnormalities in HD mice
38. shHD2.1 improves stride length in mice
39. Summary (2)
40. Acknowledgements

Topics Covered

• Brain diseases and molecular therapies
• RNA interference
• Mice models of brain disease
• Viral vectors

Links

Series:

• RNA Interference

Categories:

• Cell Biology
• Genetics & Epigenetics
• Neuroscience
• Pharmaceutical Sciences

Therapeutic Areas:

• Neurology

Talk Citation

Davidson, B.L. (2007, October 1). RNA interference for neurodegenerative diseases [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved December 3, 2024, from https://doi.org/10.69645/FEAS8148.
Export Citation (RIS)

Publication History

• Published on October 1, 2007
• Archived on June 22, 2016

Financial Disclosures

• Prof. Beverly L. Davidson has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.