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1. Introduction
2. RNA interference (RNAi)
3. RNAi: Post-Transcriptional Gene Silencing (PTGS)
4. RNAi: Transcriptional Gene Silencing (TGS)
5. dsRNA mediated TGS in S. Pombe
6. Do siRNAs directed to promoters induce TGS?
7. FIV lentiviral vector system
8. Experimental protocol
9. siRNA targeting EF1a promoter suppress GFP
10. Specificity of EF1A siRNA Mediated TGS
11. siRNA does not alter vector integration
12. siRNA mediated TGS is reversible
13. TGS is associated with DNA methylation
14. siRNA EF52 can target the EF1A promoter
15. EF1a is only inhibited at 48hrs post-transfection
16. Are intronic regions susceptible to siRNA targeting
17. Intronic region is susceptible to siRNA targeting
18. Specificity of EF315 transcriptional silencing
19. siRNA mediated TGS in human cells
20. What is the mechanism of siRNA mediated TGS?
21. TGS operates via chromatin remodeling pathway
22. siRNA mediated histone methylation
23. RNA pol II is required for siRNA mediated TGS
24. How do siRNAs find the target?
25. Detection of flag-tagged DNMTs or Hp1s
26. Specificity of DNMT binding
27. Antisense siRNA targeting HIV-1 LTR/promoter
28. Susceptibility of the HIV-1 LTR to siRNA
29. siRNA EF52 and all the factors are linked
30. Conclusions
31. Putative mechanism(s) for siRNA mediated TGS
32. Acknowledgements
33. Living on the edge

Topics Covered

• How siRNAs mediate transcriptional gene silencing (TGS) in human cells
• Histone methylation
• Dependency on active RNA polymerase II mediated transcription
• siRNA-mediated TGS
• H3K9 and H3K27 methylation of the targeted EF1A promoter
• Nuclear specific delivery of the siRNA
• Antisense strand-specific siRNA-mediated TGS of EF1A
• Targeting of the U3 region of the HIV-1 LTR/promoter
• Antisense strand of siRNA EF2 interacts with FLAG-tagged DNMT3A and the tri-methylated H3K27 methyl mark
• siRNA mediated targeting of genomic regions
• RNA Pol-II function
• Transcriptional gene silencing in human cells

Links

Series:

• RNA Interference

Categories:

• Cell Biology
• Genetics & Epigenetics

Talk Citation

Morris, K. (2007, October 1). Small interfering RNA induced transcriptional gene silencing in human cells [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved November 21, 2024, from https://doi.org/10.69645/VHCZ2546.
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Publication History

• Published on October 1, 2007
• Archived on August 13, 2020

Financial Disclosures

• Dr. Kevin Morris has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.