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1. Introduction
2. The pathway of gene expression
3. Regulatory roles for RNA
4. Defining the RNAi mechanism
5. The discovery of regulatory RNA
6. RNAi
7. dsRNA, siRNA and miRNA
8. Conversion of dsRNA to siRNA
9. Extracts from Drosophila cells and Dicer activity
10. The Drosophila Dicer family
11. Dcr-2 is essential for siRNA production
12. The initiation and effector phases
13. Biochemical definition of Drosophila RISC
14. RISC-associated proteins
15. Dcr-2 and R2D2
16. siRNAs fail to program RISC if Dcr-2 is missing (1)
17. siRNAs fail to program RISC if Dcr-2 is missing (2)
18. Native gel electrophoresis
19. Examples
20. Role of Dicers in RISC (1)
21. Role of Dicers in RISC (2)
22. mRNA become targets for destruction
23. The biological functions of RNAi
24. MicroRNAs in Drosophila
25. Polycistronic and monocistronic miRNA genes
26. Mechanism of gene silencing by miRNAs
27. Differential Gel Electrophoresis - DIGE
28. DIGE analysis of the oocyte proteome
29. The detectable proteome is repressed by miRNAs
30. miRNAs mute rather than silence most targets
31. miRNAs repress protein synthesis from mRNAs
32. Expression of genes involved in protein metabolism
33. Genes silenced by miRNAs
34. The biological function of miRNAs
35. The Drosophila ovary: stem cells in action
36. Dcr-1 mutant germ line interrupting development
37. Dcr-1 promotes division rate of germ stem cells
38. Dcr-1 promotes G1/S transition in germ stem cells
39. Dcr-1 represses p21/Dap expression
40. p21/Dap is downstream of Dcr-1 in G1/S pathway
41. Dcr-1 is not essential for division in somatic cells
42. miRNAs and stem cell growth:a model
43. Conclusions
44. Thanks

Topics Covered

• Understanding how RNAi works
• The fruitfly, Drosophila melanogaster model
• The three reasons for the success of the model
• RNAi mechanism in Drosophila and humans is similar
• Rich history of genetics in Drosophila
• Robust chemistry of the Drosophila system
• Fusion of genetics and biochemistry
• Discovery of RISC
• Discovery of Dicer
• Discovery of Ago2
• New features of RNAi biochemistry
• Greater understanding of how double-helix RNA is used to silence genes
• Development of RNAi for disease research and treatment

Links

Series:

• RNA Interference

Categories:

• Cell Biology
• Genetics & Epigenetics
• Methods

Talk Citation

Publication History

• Published on October 1, 2007
• Archived on September 28, 2017

Financial Disclosures

• Prof. Richard Carthew has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.