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1. Introduction
2. mRNA 5' cap structure
3. mRNA cap functions
4. Cap binding and translation initiation
5. Cap synthesis
6. Capping enzyme structure
7. Complementation of S.cerevisiae lacking GT
8. Recruitment of mRNA cap enzymes by P-CTD
9. P-CTD enhances guanylylation and cap formation
10. hSPT5 is a transcription elongation factor
11. SPT5 enhances guanylylation and capping
12. DSIF overcome FCP1-mediated capping decrease
13. Capping enzyme reverses repression by NELF
14. Importin-alfa
15. Imp-alfa increases MT activity
16. MT selectively binds GpppG-RNA
17. Imp-alfa enhances MT targeting to GpppG-RNA
18. Importation of nuclear proteins
19. Imp-beta blocks Imp/MT/RNA formation
20. RanGTP counteracts Imp-beta effects
21. Capping modulation
22. MT regions required for Imp-alfa binding
23. MT sequence localizes proteins to nucleus
24. MT NLS narrowed
25. Alternative NLSs in MT
26. Nuclear localized MT binds pol II
27. Capping enzyme RNAi is lethal in C.elegans
28. SiRNA inhibits expression of capping enzymes
29. Apoptosis - cleavage of caspase-3
30. RNA interference induces apoptosis
31. Inducible CE RNAi causes apoptosis
32. Summary
33. Thanks

Topics Covered

• Modification of mRNA 5' ends by addition of a m7G cap structure
• Capping for viability
• Conservation in yeast to humans
• Structure of caps and capping enzymes
• Mechanism of cap synthesis
• Protein-protein interactions that link capping to events in transcription, transport and translation
• siRNA knockdown experiments

Links

Series:

• From DNA to Proteins

Categories:

• Biochemistry
• Cell Biology

Talk Citation

Publication History

• Published on October 1, 2007

Financial Disclosures

• Prof. Aaron Shatkin has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.