Other Talks in the Series: From DNA to Proteins

The focus of this presentation is Nuclear Pre-mRNA Splicing. Splicing is an essential step in the expression of almost all genes and higher eukaryotes. We will begin by discussing the phenomenon of splicing and then discuss the mechanism by which it occurs. We will then shift to a discussion of how splicing, specifically alternative splicing, contributes to proteomic diversity. Finally, we will briefly discuss the role of splicing in messenger RNA surveillance and quality control.
This slide illustrates the structure of a typical higher eukaryotic protein coding gene. The transcribed region is shown in green and yellow, and the flanking regions are shown in red. The actual coding sequence, comprised of multiple exons, is shown in dark green. This sequence is interrupted by multiple intervening sequences, or introns, which are shown in yellow. Upon transcription, a pre-mRNA is produced which contains all of the exons and introns. To produce a functional messenger RNA, the introns must be removed by splicing, and the exons must be fused together in the order in which they were transcribed.