0:00
The focus of this presentation
is Nuclear Pre-mRNA Splicing.
Splicing is an essential
step in the expression
of almost all genes
and higher eukaryotes.
We will begin by discussing
the phenomenon of splicing
and then discuss the
mechanism by which it occurs.
We will then shift to a discussion
of how splicing, specifically
alternative splicing, contributes
to proteomic diversity.
Finally, we will briefly discuss
the role of splicing in messenger
RNA surveillance
and quality control.
0:32
This slide illustrates the
structure of a typical higher
eukaryotic protein coding gene.
The transcribed region is
shown in green and yellow,
and the flanking regions
are shown in red.
The actual coding sequence,
comprised of multiple exons,
is shown in dark green.
This sequence is interrupted by
multiple intervening sequences,
or introns, which
are shown in yellow.
Upon transcription, a
pre-mRNA is produced which
contains all of the
exons and introns.
To produce a functional
messenger RNA,
the introns must be
removed by splicing,
and the exons must be
fused together in the order
in which they were transcribed.
