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The chloroplast proteome.
In this presentation,I will review five subjects.What is proteomics and
what does it measure?Protein identification
by mass spectrometry,including intact proteins, peptide
mass fingerprinting and sequence tags.The problem of complex mixtures
and protein identification.Quantitative proteomics and
stable isotope labeling,intact protein mass measurementand 'top-down' proteomics.Let's start off by consideringwhat is proteomics and
what does it measure?
Proteomics is a component
of systems biology."We would like a computer model
that mimics life in silicoallowing accurate projections for
metabolic engineering experiments."
What exactly is proteomics?Proteomics is the scienceof describing the
complete set of proteinsexpressed from an organism's
genetic material,their interactions
with each otherand the influence
of development,environment and disease
upon their expression.Since understanding their
structure and function ofthe gene products and their
interrelationships are the overall goals,we can regard proteomics
alongside structural genomicsas a component of functional
genomics and systems biology.
The cellular proteome
is a dynamic beastcontinuously expressed
from the genomeunder the influence of the
environment and nutrient conditions.The proteome impinges back upon
the genome and back upon itselfto accomplish short-term
acclimation over minutes,medium-term acclimation
over hours and days,often involving changes
in gene expression,and ultimately accomplishing
development of growth,reproduction and evolution.
We can consider proteome flux,the flow of nutrients and
energy through transcription,translation and
protein degradationthat is controlled
by the genetic codeand numerous factors that
influence expression.Ideally, we would like to
monitor global gene expression,protein post-translational
modification and turnoverwith molecular resolution and