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Nature’s strategies in the regulation of enzyme activity by modifiers
Published on March 30, 2023 40 min
A selection of talks on Biochemistry
The ERK1/2 MAPK cascade
- Prof. Melanie H. Cobb
- University of Texas Southwestern Medical Center at Dallas, USA
Amino acid conjugation: mechanism and enzymology
- Dr. Kathleen Knights
- Flinders University, Australia
Welcome everybody to this talk on the regulation of enzyme activity by inhibitors and activators. My name is Antonio Baici, retired from the University of Zurich. I have been active in the field of enzyme kinetics, investigating the theoretical aspects of enzyme inhibition with obligations to pathological degradation of connective tissues.
In this talk, I will bring to your attention the kinetic groundwork of enzyme modifier interruptions. I will start with the general modifier mechanism and some principles of enzyme kinetics. The present nomenclature of enzyme inhibitors and activators is answered dissatisfactorally, this is partly due to the non observance of the recommendations issued by the International Union of biochemistry and Molecular Biology and partly to merging unrelated mechanisms under collected names. I will describe a systematics treatment of modifier mechanisms, which is entirely based on the recommendations and on the general modifier mechanism. Don't worry for names, concepts, and the definitions possibly new to you. You can re-examine them on links I will show in the last slide. The intended purpose of this talk is to illustrate a logical approach to the investigation of enzyme modified interactions with emphasis on allosteric regulation.
Jean Botts and her husband Manuel Francisco Morales, wrote a fundamental paper on enzyme modifier interactions. The term modify in the title declares the intention of treating every kind of enzyme inhibition and activation under the same roof. An important statement at the beginning of the mathematical derivation seems to have been underestimated by the scientific community. For the discussion of this effect, one may visualize and enzymatic patch upon the enzyme molecule, consisting of an enzymatic site which bind substrate molecules, and then adjacent side which binds modifier molecules. The highlighted adjacent side is evidently a less spectacular definition of allostatic site, published 10 years later in the famous paper by mono and co-workers. Moreover, Botts and Morales published a complete mathematical treatment, not found on all papers.