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Navigable Slide Index

1. Introduction
2. Talk outline
3. First experimental observations
4. Mirabilis crossing experiments
5. Explanations for non-Mendelian inheritance
6. Chlamydomonas mating experiments
7. Evidence for genetic determinants in chloroplasts
8. Identifying and mapping chloroplast genes
9. First complete chloroplast genome sequence
10. Organisms that retain a chloroplast
11. The chloroplast genome
12. Chloroplast DNA structure
13. Core protein genes in chloroplast DNAs (1)
14. Other chloroplast genes
15. Remnant chloroplast genomes
16. Core protein genes in chloroplast DNAs (2)
17. Organization of a typical dinoflagellate minicircle
18. Gene expression in model systems
19. Transcription in vitro from trnM2 promoter
20. Evidence for nuclear-encoded RNA polymerase
21. Transcripts in lines disrupted for chloroplast genes
22. Nuclear-encoded polymerase (NEP)
23. Class I NEP promoters
24. Post-transcriptional modification - splicing
25. Trans-splicing
26. Trans-splicing of psaA in Chlamydomonas
27. Post-transcriptional modification - editing
28. Tabacco psbL model system
29. Post-transcriptional modification - editing
30. Post-transcriptional modification - RNA turnover
31. Chlamydomonas atpB
32. Spinach petD
33. Cleavage and exonuclease activity
34. Post-transcriptional modification - RNA turnover
35. Translation
36. Why retain a chloroplast genome?
37. Requirements for functional transfer to the nucleus
38. Why retain nuclear copy?
39. Why are any genes retained in the chloroplast?
40. Chloroplast/nucleus signalling
41. Why are any genes retained in the chloroplast?
42. Summary
43. References

Topics Covered

• Early evidence for a chloroplast genome
• Organization of the chloroplast genome in plants and algae
• Gene content
• Transcription
• Roles of bacterial and phage-type RNA polymerases
• Post-transcriptional modification
• Splicing, editing and RNA turnover
• Translation
• Genome evolution
• Transfer of genes to the nucleus

Links

Series:

• Chloroplast

Categories:

• Cell Biology
• Genetics & Epigenetics
• Plant & Animal Sciences

Talk Citation

Howe, C. (2007, October 1). The chloroplast genome and chloroplast gene expression [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved April 15, 2025, from https://doi.org/10.69645/BAUT8611.
Export Citation (RIS)

Publication History

• Published on October 1, 2007
• Archived on May 31, 2022

Financial Disclosures

• Prof. Christopher Howe has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.