We noted you are experiencing viewing problems
-
Check with your IT department that JWPlatform, JWPlayer and Amazon AWS & CloudFront are not being blocked by your network. The relevant domains are *.jwplatform.com, *.jwpsrv.com, *.jwpcdn.com, jwpltx.com, jwpsrv.a.ssl.fastly.net, *.amazonaws.com and *.cloudfront.net. The relevant ports are 80 and 443.
-
Check the following talk links to see which ones work correctly:
Auto Mode
HTTP Progressive Download Send us your results from the above test links at access@hstalks.com and we will contact you with further advice on troubleshooting your viewing problems. -
No luck yet? More tips for troubleshooting viewing issues
-
Contact HST Support access@hstalks.com
-
Please review our troubleshooting guide for tips and advice on resolving your viewing problems.
-
For additional help, please don't hesitate to contact HST support access@hstalks.com
We hope you have enjoyed this limited-length demo
This is a limited length demo talk; you may
login or
review methods of
obtaining more access.
Printable Handouts
Navigable Slide Index
- Introduction (1)
- Introduction (2)
- Contents
- Graphical conventions for glycans
- Composition of yeast cell walls
- Overall architecture of yeast cell walls
- Yeast glycan structures
- Chitin
- Chitin synthesis
- Structure and biosynthesis of β1,3 glucan
- β1->3 glucan synthesis
- Branched β1,6 glucan: structure and biosynthesis mystery
- β1->6 glucan crosslinks other wall components
- Localization of different cell wall proteins
- Mannoproteins (1)
- Candida albicans Als adhesins (1)
- Mannoproteins (2)
- Secretory pathway for GPI-crosslinked wall proteins in yeast
- Biosynthesis of N-glycans
- Biogenesis of GPI-mannoprotein-glucan crosslinks
- Pir protein glutamyl-glucan esters
- Proposed mechanism for crosslink between N-mannan and β-glucan
- Recap: mannoprotein crosslinks to glucan
- Where are we now? (1)
- Where are we now? (2)
- Dynamics: response to cell wall stress
- Dynamics (1)
- Dynamics (2)
- Candida albicans Als adhesins (2)
- Mechanism of formation of cell surface amyloid-like mannoprotein clusters
- Minute-by-minute dynamics in walls
- C. albicans biofilm
- Generality and evolution
- Complex immune responses to cell wall patterns and antigens
- Recap
- Bibliography (1)
- Bibliography (2)
- Thank you for listening
Topics Covered
- Fungal cell walls
- Glycans
- S. cerevisiae
- C. albicans
- Mannose
- Chitin
- Glycan biosynthesis
- Mannoproteins
- GPI-crosslinked proteins
- N. crassa
- Yeast cell wall dynamics
- Cell wall conservation and evolution
Links
Series:
Categories:
Therapeutic Areas:
Talk Citation
Lipke, P. (2022, November 30). Structure of cell walls in yeast and other fungi [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved December 10, 2024, from https://doi.org/10.69645/BAGG2309.Export Citation (RIS)
Publication History
Financial Disclosures
- Dr. Peter Lipke, Grant/Research Support (Principal Investigator) holds a grant from Biothera, the Immune Health Company. Their products are neither mentioned nor promoted in the lecture.
A selection of talks on Infectious Diseases
Transcript
Please wait while the transcript is being prepared...
0:00
Hello, my name is
Peter Lipke from
the Department of Biology at
Brooklyn College of the City
University of New York.
Today I'm going to
talk about glycans and
glycoproteins in the cell walls
of yeast and other fungi.
0:16
Fungal cell walls
are not well known,
but they are the
cellular equivalent of
the extracellular
matrix in animal cells.
They have many of
the same functions,
including protection
of the cell surface
and interaction with
the environment.
We're going to talk
about the structure and
biosynthesis of the
components of the cell walls
and about relatively
recent discoveries
about how the cell walls
are assembled and modified.
On this slide, you see some
electron micrographs of
yeast cell walls, mostly with
the extracellular
environment at the top.
The visible structures
are the walls.
The membranes are not visible
in these micrographs,
but you can see the
cytoplasm interior
to the membrane
towards the bottom.
On the left to the
slide, you see
a cartoon of the arrangement
of the components of
the cell wall and we'll
use this cartoon to
orient ourselves as we
go through the lecture.
If you look at the
bottom of the slide,
there are the reference numbers
for this particular slide.
The last two slides are
a bibliography with
numbered references,
so you can access the
relevant literature.
1:21
This lecture will
have eight sections.
First, we will talk about
graphic conventions for
how we depict the glycans.
Next, we will talk about
the overall architecture
of yeast cell walls.
The third section will
include the structure and
biosynthesis of individual
components of the wall.
Then, we will have a section
about the assembly
and cross-linking
of all of these components
into a macromolecular complex.
Fifth, we will talk about
how the wall is modified to
accommodate both
environmental needs
and the needs of cellular
differentiation.
Sixth is a brief
discussion about how
general the model is across
the evolution of fungi.
Seventh is a one-slide
summary of interactions
between wall components and
the mammalian immune system,
a subject that would be
a separate series of
lectures based on
thousands of papers.
Finally, there is a brief
recap and bibliography.