Printable Handouts

PDF

Navigable Slide Index

1. Introduction
2. HIV
3. Cell cycle progression and retrovirus replication
4. Resting T cells and HIV
5. Link between uncoating and PIC nuclear import
6. Preintegration complex (PIC)
7. Intracellular transport of PICs
8. The HIV-1 PIC has multiple components with NLS
9. HIV-1 Vpr vs. HIV-2 Vpx
10. HIV-1 Vpr interaction factors
11. Cellular proteins that influence PIC import or action
12. HIV-2
13. HIV-2: model system for PIC nuclear import
14. Vpx cellular localization
15. Cellular localization of LacZ-fusions (1)
16. Cellular localization of LacZ-fusions (2)
17. Alignment of HIV-2/SIV Vpx amino acids 61-79
18. Identifying residues critical for PIC nuclear import
19. Nuclear localization signal of HIV-2 Vpx
20. Vpx interaction with Hsp40
21. Heat shock proteins
22. Heat shock proteins and viruses
23. Vpx interacts with Hsp40/DnaJB6 (1)
24. Vpx interacts with Hsp40/DnaJB6 (2)
25. DnaJB6 over expression and Vpx packaging
26. Hsp40/DnaJB6 enhances Vpx nuclear localization
27. DnaJB6 down regulation and PIC nuclear import
28. Novel mechanism for Vpx-mediated PIC import
29. Nuclear import inhibitors

Topics Covered

• Role of cell cycle progression in retrovirus replication
• Resting T-cells and HIV
• Uncoating and PIC nuclear import
• Preintegration complex (PIC)
• Intracellular transport of PIC
• HIV-1 Vpr vs. HIV-2 Vpx
• HIV-1 Vpr interaction factors
• HIV-2: model system for PIC nuclear import
• Vpx cellular localization
• Ability of Vpx-NLS mutant viruses to infect arrested and dividing U937 cells
• Nuclear localization signal of HIV-2 Vpx
• Vpx interaction with HSP 40
• Heat shock proteins and viruses
• Over-expression of DNAJB6 causes reduction of Vpx packaging in VLP
• Down regulation of DNAJB6 reduces nuclear import of HIV-2 PIC
• Novel mechanism of Vpx-mediated PIC import

Links

Series:

• Retroviruses

Categories:

• Biochemistry
• Cell Biology
• Microbiology

Therapeutic Areas:

• Infectious Diseases

Talk Citation

Publication History

• Published on October 1, 2007
• Archived on December 10, 2018

Financial Disclosures

• Prof. Lee Ratner has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.