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- Introduction to Protein Folding and Misfolding
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69 min
1. Protein folding and misfolding: from theory to therapy- Prof. Christopher Dobson
- Stability and Kinetics of Protein Folding
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55 min
2. Mechanisms of protein folding reactions- Prof. Thomas Kiefhaber
- Protein Folding Theory
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53 min
3. Mapping disordered proteins with single-molecule FRET- Dr. Hagen Hofmann
- Protein Folding Simulations
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36 min
4. Protein folding- Prof. Eugene Shakhnovich
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42 min
5. Simulating protein folding with full atomistic detail- Prof. Vijay Pande
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58 min
6. Molecular dynamics simulations of protein dynamics, unfolding and misfolding- Prof. Valerie Daggett
- Protein Folding Inside the Cell: Chaperones
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42 min
7. Protein folding Inside the cell: macromolecular crowding and protein aggregation- Prof. Emeritus R. John Ellis
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34 min
8. Chaperone mechanisms in cellular protein folding- Prof. Dr. F. Ulrich Hartl
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60 min
9. Quality control of proteins mislocalized to the cytosol- Dr. Ramanujan Hegde
- Protein Misfolding and Disease
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44 min
- Protein Design
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33 min
11. Designing proteins with life sustaining activities 1- Prof. Michael Hecht
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24 min
12. Designing proteins with life sustaining activities 2- Prof. Michael Hecht
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48 min
13. Folding and design of helical repeat proteins- Prof. Lynne Regan
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44 min
14. Design and engineering of zinc-finger domains- Prof. Jacqui Matthews
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47 min
15. Prediction and design of protein structures and interactions- Prof. David Baker
- Amyloid Fibrils: Structure, Formation and Nanotechnology
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39 min
16. Amyloid fibrils as functional nanomaterials- Prof. Juliet Gerrard
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37 min
17. Functional amyloid fibrils from fungi and viruses- Prof. Margaret Sunde
- Intrinsically disordered Proteins
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40 min
18. Fuzzy protein theory for disordered proteins- Prof. Monika Fuxreiter
- Intersection of RNA, translation and protein aggregation.
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47 min
19. Expanding roles of RNA-binding proteins in neurodegenerative diseases- Prof. Aaron D. Gitler
- Proteostasis
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49 min
20. Adapting proteostasis to ameliorate aggregation-associated amyloid diseases- Dr. Jeffery W. Kelly
- Archived Lectures *These may not cover the latest advances in the field
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60 min
21. Amyloidosis: disease caused by amyloid- Prof. Mark Pepys
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34 min
22. Protein folding and dynamics from single molecule spectroscopy- Prof. Dr. Benjamin Schuler
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41 min
23. Prion diseases- Prof. Fred Cohen
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31 min
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38 min
25. Titin I27: a protein with a complex folding landscape- Dr. Jane Clarke
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49 min
26. Novel proteins from designed combinatorial libraries- Prof. Michael Hecht
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47 min
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35 min
28. The sequence determinants of amyloid fibril formation- Prof. Fabrizio Chiti
Printable Handouts
Navigable Slide Index
- Introduction
- Libraries of de novo amino acid sequences
- Designed libraries of folded proteins
- Amphipathic alpha helix and beta strand
- Binary patterned 4-helix bundle
- Suggested sequence for alpha-helix
- Genetic code for the suggested sequence
- Rational design and combinatorial approach
- Cloning the library into E.coli
- Example of several sequences from the library
- Combinatorial libraries of sequences
- Well folded "native-like" structures
- Most of the proteins were molten globules
- Design: 74 amino acid, 14 residues/helix
- The first and the second generation libraries
- Stability of the 1st and 2nd generation libraries
- NOESY spectra
- NMR for proteins from the unselected library
- Experimentally determent structure
- Superposition of low energy structures
- Polar/nonpolar periodicity of de novo 4-helix bundle
- Sequence of S-824
- Functional proteins in unselected binary libraries
- Heme binding
- Functions of heme proteins
- Peroxidase reaction
- Screen for peroxidase activity
- Peroxidase activity by oxidation of ABTs
- Catalysis without cofactors?
- Hydrolysis of p-nitrophenyl acetate by S-824
- Esterase activity kcat/kuncat = 8700
- A binary code for protein design
- Binary patterning of alpha-helix and beta strand
- A designed beta sheet template
- Binary patterned beta-sheet sequences
- Alpha-helix versus beta-sheet
- De novo beta-sheet proteins
- Binary patterns in natural sequences
- Engineering monomeric beta-sheet proteins
- Chromatography and circular dichroism
- Summary for binary code for protein design
- Biomaterials
- Binary patterned design for beta-sheet proteins
- Model of graphite surface and AFM image
- Acknowledges
Topics Covered
- Novel proteins can be devised using either rational/computational approaches or random/combinatorial methods
- Description of a hybrid approach that combines rational and combinatorial methods: libraries of novel proteins
- Combinatorial diversity
- Random vs. focused libraries
- Amphiphilic alpha-helices and beta-strands
- Binary patterning of polar and non-polar amino acids
- Genetic encoding of combinatorial mixtures of polar and non-polar amino acids
- Design of libraries of novel 4-helix bundles
- Library construction and expression in vivo
- Dynamic vs. wellordered protein structures
- Second generation libraries
- Biophysical characterization
- Solution structure of native-like 4-helix bundle proteins from designed libraries
- Functionally active proteins including cofactor binding proteins, peroxidases and esterases
- Designed libraries of beta sheet proteins: amyloid-like fibrils, monomeric beta-sheet proteins and novel biomaterials
Talk Citation
Hecht, M. (2007, October 1). Novel proteins from designed combinatorial libraries [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved November 22, 2024, from https://doi.org/10.69645/UTMA5950.Export Citation (RIS)
Publication History
Financial Disclosures
- Prof. Michael Hecht has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.
