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Printable Handouts
Navigable Slide Index
- Introduction
- Ca2+ and cAMP: two major 2nd messengers
- Outline
- Ca2+ sensitivities of adenylyl cyclases in vitro
- Adenylyl cyclase type 8 (AC8)
- Ca2+ sensitivities of adenylyl cyclases in vitro
- In vitro regulation by Ca2+ is mimicked
- Capacitative Ca2+-entry
- Ca2+-regulation of ACs is selective for CCE
- Capacitative Ca2+ entry exclusively activates AC8
- Adenylyl cyclases and CCE channels colocalize
- Ca2+-sensitive cyclases are enriched in lipid rafts
- Cyclase localization in caveolae/rafts
- Regulation/localization of ACs
- Cyclase presence in rafts
- Probing of cyclase raft-targeting
- Cyclese trafficking to plasma membrane
- Role of TM vs. cytosolic domains
- Hypothetical calcium signal-coupling domain
- Structure of K ATP channel
- Roles of the N-terminus of AC8
- Calmodulin binding sites
- Essential component of responsivness to CCE
- 2nd role for AC8 N-terminus binding of PP2A
- Another element of the AC microdomain
- pH sensitivity of Ca-sensitive Acs
- Effect of modest in vitro pH changes
- Global pH changes don't affect stimulation of AC8
- Global pH changes don't affect stimulation of AC6
- NHE1 activity minimizes the effects of weak acid
- NHE1 is located in lipid rafts
- NHE1 and ACs
- Microdomains of cAMP
- Agonist evoked cAMP changes
- Phosphodiesterase activity
- Anchoring proteins regulate cAMP dynamics
- Questions about AKAP and PDE4
- Candidate membrane-associated AKAPs
- RNAi of candidate AKAPs
- Co-immunoprecipitation of gravin with PDE4D
- Signalling module in HEK 293 cells
- The interplay between Ca2+ and cAMP signals
- CCE-induced stimulation of AC8 activity
- Synchronous Ca2+ and cAMP oscillations
- Recovery from Ca2+-evoked cAMP changes
- Agonist-induced oscillations in Ca2+ and cAMP
- Sequence of events that drive cAMP oscillations
- What constitutes a microdomain for cAMP or AC?
- The cAMP microdomain
- Conclusions
- The key supporting players
Topics Covered
- Calcium-entry
- Elements of the compartment: adenylyl cyclases, lipid rafts, PP2A, NHE1, calmodulin and PDE
- cAMP kinetics within the compartment in single cells: role of PDE4, PKA and AKAPs
- cAMP oscillations
Talk Citation
Cooper, D. (2007, October 1). Calcium-regulated adenylyl cyclases and cyclic AMP compartmentalization [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved December 7, 2024, from https://doi.org/10.69645/QRUW4225.Export Citation (RIS)
Publication History
Financial Disclosures
- Prof. Dermot Cooper has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.
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