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Printable Handouts
Navigable Slide Index
- Introduction
- Outline
- 1879: Discovery of basophils
- Basophils are widespread in the animal kingdom
- The basophil count (1)
- The basophil count (2)
- Which factors govern basophil numbers in peripheral blood?
- Development of basophils
- IL-3 and basophils
- Morphological appearance of basophils
- Basophil granules: metachromasia
- What do basophil granules contain?
- Basogranulin (BB1 antigen)
- Basophil surface: chemokine receptors
- Basophil surface: selectins and integrins
- Basophils enter tissues during allergic responses
- Mechanisms of basophil activation
- IgE/FcεRI-dependent activation (1)
- FcεRI and serum IgE
- IgE/FcεRI-dependent activation (2)
- Basophil activation by allergens
- Early events in signal transduction
- Non-releasers
- Kinetics of mediator induction
- Kinetics of cytokine induction
- Differential priming effects of IL-3 on IL-4/histamine and IL-13
- Which factors govern basophil activation?
- Non IgE-dependent activation
- Basophil surface: activation
- Basophil activation by proteases
- Human peripheral blood basophils do not express PAR mRNAs
- PAR-1, -2 and -4 agonists do not induce IL-4/IL-13 from human PB basophils
- Lectin activation of basophils (1)
- Lectin activation of basophils (2)
- Role of basophils in the late phase allergic respons
- Role of basophils in the late phase response
- Late phase response (LPR)
- Indirect evidence for basophil role in LPR
- Basophil activation tests
- CD63 and CD203c are used for Basophil Activation Tests (BAT)
- Key points to remember
- Basophils are ‚in‘!
- What about mouse basophils?
- Mouse basophils
- Human vs murine basophils
- Functional differences between human and murine basophils (examples)
- Summary: human basophil involvement in disease
Topics Covered
- Human peripheral blood basophils
- The basophil count
- Basophil growth and development
- Surface receptors (chemokine receptors, selectins, integrins)
- Activation mechanisms (IgE dependent and non-IgE dependent)
- Surface activation markers and basophil activation tests
- The role of basophils in late phase response
- Differences between murine and human basophils
Links
Series:
Categories:
Therapeutic Areas:
Talk Citation
Falcone, F.H. (2020, March 29). The role of basophils in allergy [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved December 13, 2024, from https://doi.org/10.69645/CJQD9100.Export Citation (RIS)
Publication History
Financial Disclosures
- Dr. Franco Falcone has no commercial/financial relationships to disclose.
Other Talks in the Series: Allergy - From Basics to Clinic
Transcript
Please wait while the transcript is being prepared...
0:00
Hello. My name is Franco Falcone.
Today, I would like to speak about "The Role of Basophils in Allergy".
This is a cell type which despite having been discovered almost 140 years ago,
has since intrigued scientists.
It was only after the discovery that basophils are the source of histamine in blood and
the discovery of immunoglobulin E some
50 years ago that we began to understand their function.
This lecture will cover
the basic biological properties of basophils and their roles in allergy.
0:32
So this is the outline of my talk.
I'm going to talk about the basophil development and the morphology.
I'm then going to talk briefly about
how the number of basophils are counted in peripheral blood.
I will then move on to talk about the surface of basophils.
I will describe the cytokine receptors,
the growth factor receptors,
the chemokine receptors, and the selectins and integrins on their surface.
I will then move on and describe basophil activation mechanisms,
starting with IgE-dependent activation mechanisms,
then I will move on to alternative mechanisms.
I will talk about mediators and how these mediators are measured.
I will mention basophil activation tests.
Finally, I will put all of this
together and I will discuss the role of basophils in allergy.
Mainly I will be talking about late phase reaction.
I will close the presentation with some differences between mouse and human basophils.
1:35
Basophils were discovered in 1879 by Paul Ehrlich.
One year earlier, Ehrlich had described mast cells in connective tissues.
Basophils and mast cells both show
similar staining patterns of cytoplasmic granules when stained with basophilic dyes.
Despite having been discovered such a long time ago,
it took almost a 100 years before
the secrets behind the cell type started becoming unraveled.
Basophils are widespread in the animal kingdom.