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1. Introduction
2. Ca regulates cell function using Ca sensors
3. How do calcium sensors work?
4. Calcium binding to proteins in vitro
5. The neuronal calcium sensor (NCS) family
6. High similarity of the class B NCS proteins
7. Properties of the NCS proteins
8. The structure of NCS-1
9. The Ca/myristoyl switch
10. Structural states of recoverin
11. Recoverin structure and function
12. Hippocalcin, not NCS-1 has a Ca/myristoyl switch
13. Translocation of hippocalcin-EYFP
14. Dynamics of hippocalcin-EYFP translocation
15. Ca dependency of hippocalcin-EYFP translocation
16. The hippocalcin Ca/myristoyl switch in neurons
17. Properties of the NCS proteins:specific localization
18. Distinct localization of KChIP1 and NCS-1
19. The myristoylation motif of KChIP2
20. Myristoylation motif determines localization
21. Myr-hippocalcin/phosphoinositides interactions
22. NCS protein activation
23. Functions of the NCS proteins
24. Frequenin and synaptic efficacy
25. Functions of NCS-1 from genetic studies
26. Differential effects of NCS-1 on exocytosis
27. Effects of NCS-1 on signaling pathways
28. Functions of NCS-1 from non-genetic studies
29. Function of hippocalcin in synaptic plasticity
30. Class E NCS proteins
31. KChIP interaction with Kv4 K channels
32. KChIP1 stimulation of Kv4 traffic
33. Molecular targets of the NCS proteins
34. Characterized target proteins of NCS-1
35. Binding of biotinylated myr-NCS-1
36. Identification of new targets for NCS proteins
37. Target proteins for NCS-1
38. NCS, CaBP1 and calmodulin targets
39. Summary and conclusions
40. Acknowledgements
41. Appendix: references (1)
42. Appendix: references (2)
43. Further reading

Topics Covered

• Introduction to calcium sensors
• The neuronal calcium sensor (NCS) protein family
• The calcium/myristoyl switch
• Dynamics of hippocalcin translocation
• Specific localization of NCS proteins
• Functions of NCS-1
• Hippocalcin in synaptic plasticity
• KChIP proteins in traffic of potassium channels
• Molecular targets of NCS proteins
• The unique features of the NCS family

Links

Series:

• Calcium Signaling I

Categories:

• Biochemistry
• Cell Biology
• Neuroscience

Talk Citation

Burgoyne, B. (2010, November 1). Neuronal calcium sensor proteins: calcium sensors and regulators of diverse cellular functions [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved April 15, 2025, from https://doi.org/10.69645/CJPW9657.
Export Citation (RIS)

Publication History

• Published on October 1, 2007
• Updated on November 1, 2010

Financial Disclosures

• Prof. Bob Burgoyne has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.