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1. Introduction
2. Endoplasmic reticulum
3. Mitochondria
4. How to measure organelle Ca?
5. Cameleon probes for Ca measurements (1)
6. Cameleon probes for Ca measurements (2)
7. ApoK1er: a probe for Ca measurements
8. Ca dynamics in the cytosol, ER and mitochondria
9. Ca crosstalk between the ER and the mitochondria
10. Importance of mitochondrial Ca
11. Ca regulation of mitochondrial ATP biosynthesis
12. Principle parameters for the Ca flux
13. Biophysical conditions: membrane potential
14. FCCP depolarizing the membrane
15. Effect of depolarizing agent on Ca flux
16. Morphological aspects: interorganelle distance
17. Impact of interorganelle distance of Ca crosstalk
18. Morphological aspects: interacting surface area
19. Colocalization of ER and mitochondria
20. Morphological aspects: organelle integrity
21. Organelle integrity - analyses
22. Biological keystone: IP3 receptor, VDAC
23. Biological keystone: mitochondrial uniporter
24. Mitochondrial uniporter - references
25. Functional parameters: mitochondrial uptake
26. Phases of mitochondrial uptake
27. The extrusion phase - NCX
28. The extrusion phase - SERCA pump
29. Mitochondrial Ca extrusion is coupled with SERCA
30. Mitochondria fragmentation effect on Ca crosstalk
31. Mitochondria fragmentation delays ER Ca refilling
32. The transit phase (1)
33. The Ca paradox
34. Inner high Ca inhibits Ca/K pathway
35. Ca/K channel dramatically affects Ca entry
36. Subplasmalemmal Ca gradients establishment
37. Measuring local Ca gradients beneath the PM
38. Measuring with patch clamp
39. ER and mitochondria create opposite Ca gradients
40. The transit phase (2)
41. Interorganelle Ca crosstalk
42. Thank you

Topics Covered

• 3D structure of the endoplasmic reticulum and mitochondria
• Techniques to measure organelle calcium
• Mitochondrial functions
• Principles of mitochondrial calcium signaling
• Functional coupling of the endoplasmic reticulum and the mitochondria
• Consequences of alterations in the structural integrity of the mitochondria
• Subplasmalemmal mitochondrial calcium buffering
• Physiological importance of mitochondrial calcium uptake
• The cellular calcium cycle

Links

Series:

• Calcium Signaling I

Categories:

• Biochemistry
• Cell Biology

Talk Citation

Graier, W. (2007, October 1). Ca2+ dynamics between mitochondria and the endoplasmic reticulum [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved April 15, 2025, from https://doi.org/10.69645/AXJM4702.
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Publication History

• Published on October 1, 2007

Financial Disclosures

• Dr. Wolfgang Graier has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.