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Ladies and gentlemen,
dear colleagues.
Antonio Baici from the
University of Zurich.
I guide you through an excursion
in the world of kinetics,
commented with Italian accent.
The purpose of my talk is to
discuss some topics and methods
of practical interest for the
kinetic investigation of proteins
interacting with other proteins,
ligands, and metabolites
in general, with emphasis on
the kinetics of enzyme action.
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Biochemical kinetics
deals with the study
of the velocity of biologically
relevant processes.
It represents an
indispensable support
for understanding the mechanism
of action of proteins.
In fact knowing how fast
an event takes place
gives useful information
about its character.
We are aware that kinetics alone
cannot answer all questions
on the mechanism of action
of the biological process.
However, pulled information from
kinetics, structured analyses,
site-directed mutagenesis, and
other methods will do the job.
1:01
I'd like to talk about a few
concepts of practical interest.
First, I'll give you some background
on different kind of information
provided by thermodynamics
and kinetics.
Then I'd like to talk about
the kinetics of enzyme action,
starting from catalysis.
After introducing basic
methods of investigation,
I will describe two approaches
for writing kinetic equations.
Understanding the [INAUDIBLE]
of the entire progress
of an enzyme-catalyzed
reaction is a central issue
for getting a clear picture of
what is going on in kinetics.
I also try to convince you that
graphical analysis of kinetic data
is not outmoded.
With the correct use of
it, statistical analysis
using mathematical methods
explained by a powerful partner
for solving complex problems.
I conclude my talk with a
discussion of some aspects
of enzyme inhibition, focusing
on practical methods of analysis.
