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1. Introduction
2. Ca2+- modulated functions in mammalian cells
3. Ca2+ -binding proteins
4. Ca2+ -transporting proteins
5. Plasma membrane Ca2+ pump (PMCA pump)
6. PMCA pump - structure
7. PMCA pump - calmodulin binding
8. Activation of PMCA pump by calmodulin (CaM)
9. The four basic isoforms of PMCA pump
10. Main properties of the four basic pump isoforms
11. Splicing isoforms of PMCA pump
12. PMCA4 splicing isoforms: CaM affinity
13. PMCA3 splicing isoforms: CaM affinity
14. The isoforms interact with various partners
15. Interaction of PMCA with the 14-3-3-epsilon protein
16. The 14-3-3-epsilon proteins
17. PMCA4 pulls down only 14-3-3-epsilon
18. 14-3-3-epsilon overexpression or silencing
19. 14-3-3-epsilon overexpression: PMCA 2 vs. 4
20. Calcium signaling and disease
21. Reminder: splicing isoforms of PMCA pump
22. PMCA2 expression
23. The organ of Corti
24. Ca2+ modulate the current generated by hair cells
25. PMCA2 is expressed specifically in the stereocilia
26. Alternative splicing of PMCA2
27. Immunolocalization of different PMCA2 isoforms
28. Differences in the activity of PMCA2 isoforms
29. Effects of mutations in the PMCA2 gene
30. PMCA2wa mutations linked to deafness
31. Immunolocalization of PMCA2 mutants
32. Differences in the activity of mutated PMCA2 (1)
33. Differences in the activity of mutated PMCA2 (2)
34. Immunolocalization of mutated PMCA2 variants
35. Acknowledgements

Topics Covered

• Targets of the signaling function of calcium
• Decoding of the calcium message by specific calcium binding proteins
• Control of calcium in the cell by calcium transporting proteins in the membranes
• Calcium pumps: biochemistry, molecular biology, structure and regulation
• Similarities in the regulation mechanisms between the two major pumps, that in the endoplasmic reticulum and that in the plasma membrane
• Ambivalence of the calcium signal
• Calcium signaling defects and related pathologies
• Genetic defects of the plasma membrane calcium pumps in hereditary deafness

Links

Series:

• Calcium Signaling I

Categories:

• Biochemistry
• Cell Biology

Talk Citation

Carafoli, E. (2007, October 1). The plasma membrane calcium pump: biochemistry, physiology and molecular pathology [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved April 15, 2025, from https://doi.org/10.69645/OZMR4561.
Export Citation (RIS)

Publication History

• Published on October 1, 2007
• Archived on February 25, 2021

Financial Disclosures

• Prof. Ernesto Carafoli has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.