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1. Introduction
2. Calcium is an ubiquitous messenger
3. Spatial control of calcium
4. Calcium-reporting dyes
5. Basic mechanisms of calcium regulation
6. Why have internal calcium stores and release?
7. Some organelles that serve as calcium stores
8. Ca mobilization by hormones/ neurotransmitters
9. Calcium mobilizing messengers
10. Intracellular signaling by IP3
11. Structures of calcium mobilizing messengers
12. Multiple Ca release mechanisms
13. cADPR activity was discovered in sea urchin eggs
14. Studying Ca release in cell-free systems
15. Ca release in urchin egg homogenates
16. Role of cADPR in calcium signaling
17. Second messenger roles for cADPR
18. cADPR/NAADP synthesis, analogues and drugs
19. Synthesis of cADPR and analogues
20. Caged cADPR photolysis induces Ca waves
21. Caged Ca photolysis evokes local Ca rise
22. Potentiation of CICR by cADPR
23. Ryanodine receptors: function
24. Ryanodine receptors: imaging elementary events
25. Photolysis released cADPR and Ca transients
26. cADPR antagonist abolishes Ca waves
27. cADPR increases the frequency of Ca sparks
28. Mechanism of enhanced Ca release by cADPR
29. Isoprenaline-evoked increases in cADPR levels
30. Pharmacology of cADPR increases
31. Role of cADPR in cardiac E-C coupling
32. Summary of cADPR activity
33. cADPR in Ca signaling
34. What is NAADP
35. Photolysing caged NAADP results in a Ca wave
36. Spatial sensitivity to low [NAADP]
37. Effect of CICR channels inhibition
38. NAADP: spatial/ temporal aspects of Ca signals
39. NAADP stores are separate from the ER
40. Stratification of sea urchin eggs
41. GPN rupture lysosomes
42. Lysotracker
43. GPN ruptures lysosomes and releases Ca
44. NAADP is an initiator: Ca-induced Ca release
45. NAADP releases Ca from lysosomal stores
46. Organelle marker enzymes
47. Receptor distribution
48. Ca uptake into NAADP-sensitive stores
49. NAADP: special channel or special regulation?
50. Inhibition of NAADP-induced Ca release
51. NAADP-induced calcium release
52. NAADP-induced Ca release amplification by RyR
53. Bafilomycin inhibits NAADP-induced Ca release
54. Interactions between NAADP and CICR channels
55. Auto-inactivation of NAADP-induced Ca release
56. Specificity of receptor-mediated NAADP signaling
57. NAADP triggers global Ca signals
58. CCK-evoked calcium spiking blocked by GPN
59. CCK-evoked calcium spiking blocked: bafilomycin
60. Selectivity in GPN blockage
61. Radio-receptor assay for NAADP levels
62. CCK-induced changes in NAADP / cADPR levels
63. NAADP: trigger hypothesis
64. Receptor specificity in pancreatic acinar cells
65. ARC can synthesize cADPR and NAADP
66. NAADP changes in CD38-/- mouse
67. NAADPR is easily solubilized as a native protein
68. Affinity purification of the NAADP receptor
69. NAADP binding proteins
70. Summary

Topics Covered

• Calcium signaling mechanisms
• Calcium stores and calcium mobilization
• Intracellular calcium release channels
• cADPR as an intracellular messenger
• Ryanodine receptors
• Cardiac excitation
• Coupling
• NAADP
• Acidic calcium store
• Smooth muscle cell calcium signaling
• Calcium spiking
• NAADP receptors

Links

Series:

• Calcium Signaling I

Categories:

• Biochemistry
• Cell Biology

Talk Citation

Galione, A. (2007, October 1). cADPR and NAADP: messengers for calcium signalling [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved November 21, 2024, from https://doi.org/10.69645/MZOM2005.
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Publication History

• Published on October 1, 2007

Financial Disclosures

• Prof. Antony Galione has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.