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Navigable Slide Index

1. Introduction
2. SGPB
3. Pioneers of protein crystallography
4. How to solve a crystal structure in a nutshell
5. Crystallization by vapour diffusion
6. More crystals
7. Seven crystal classes and fourteen Bravais lattices
8. Space groups in 2D
9. Plane group
10. Data collection - crystal mounting
11. Intensity data collection
12. Advanced photon source, Argonne
13. Advanced photon source
14. Diffraction pattern of myoglobin
15. Diffraction from a crystal of bluetongue virus
16. How to convert the measured intensities
17. Electron density equation
18. The Harker construction
19. The Patterson function
20. Deduction of coordinates from Patterson map
21. Multiple isomorphous replacement
22. A second derivative
23. Computing the protein's electron density
24. Fitting the density with a model
25. Fitting strands of a beta-sheet
26. Programs to assist fitting
27. Fitting side chains to density
28. The final model after refinement
29. Quality of the intensity data
30. Quality of the final structure
31. Polypeptide conformational angles, phi and psi
32. Ramachandran plot of phi vs. psi
33. Major advances in macromolecular crystallography
34. Acknowledgments

Topics Covered

• Protein crystallography
• Protein crystallization methods
• Crystal symmetry
• X-ray intensity data collection
• Electron density equation
• The Patterson function
• Multiple isomorphous replacement phasing
• Fitting the electron density with a model
• Refinement of the model
• Estimating quality of the model

Links

Series:

• Protein Epidemiology

Categories:

• Biochemistry
• Cell Biology
• Methods

Talk Citation

Publication History

• Published on October 1, 2007
• Archived on June 22, 2016

Financial Disclosures

• Prof. Michael James has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.