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- Introduction to Calcium Signaling
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1. Introduction to cellular calcium signaling
- Dr. Martin Bootman
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2. Monitoring Ca2+ concentration in living cells
- Dr. Marisa Brini
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3. Cell boundary theorem
- Prof. Eduardo Ríos
- Calcium Influx
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4. Arachidonic acid and store-independent Ca2+ entry
- Dr. Luca Munaron
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5. Voltage-dependent calcium channels
- Prof. Annette Dolphin
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7. Intracellular Ca2+ signaling: calcium influx
- Prof. Anant Parekh
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8. Molecular identification of the CRAC channel
- Prof. Michael Cahalan
- Calcium Release
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10. The InsP3 receptor calcium release channel
- Prof. J. Kevin Foskett
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11. Molecular biology of ryanodine receptors: an overview
- Dr. Christopher George
- Prof. F. Anthony Lai
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12. cADPR and NAADP: messengers for calcium signalling
- Prof. Antony Galione
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13. Ryanodine receptors and cardiac function
- Prof. David Eisner
- Calcium Efflux and Sequestration
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14. Sodium-calcium exchange
- Prof. John Reeves
- Organelle Calcium
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15. Regulation and role of mitochondrial Ca2+ homeostasis
- Prof. Rosario Rizzuto
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16. Peroxisomes and Golgi apparatus as players in Ca2+ homeostasis
- Dr. Paola Pizzo
- Dr. Alex Costa
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17. Ca2+ dynamics between mitochondria and the endoplasmic reticulum
- Dr. Wolfgang Graier
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18. Nuclear calcium signaling
- Dr. Oleg Gerasimenko
- Dr. Julia Gerasimenko
- Spatiotemporal Calcium Signals
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19. Regulation of intracellular calcium signaling, localized signals and oscillations
- Prof. Barbara Ehrlich
- Calcium Effectors
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24. Calcium-regulated adenylyl cyclases and cyclic AMP compartmentalization
- Prof. Dermot Cooper
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25. Calcium and transcription-coupling
- Dr. Karen Lounsbury
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26. Cellular calcium (Ca2+) buffers
- Prof. Dr. Beat Schwaller
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27. Extracellular calcium signaling
- Dr. Aldebaran M. Hofer
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28. Ca2+, fertilization and egg activation
- Prof. Karl Swann
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29. Calcium regulation of transcription in plants
- Prof. Hillel Fromm
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30. Mechanisms regulating STIM expression and function in Ca2+ signaling
- Dr. Jonathan Soboloff
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31. Dynamic signal encoding in the S. cerevisiae calcium response
- Dr. Chiraj Dalal
- Calcium and Disease
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32. Polycystins, calcium signaling and pathogenesis of polycystic kidney disease
- Prof. Laura del Senno
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33. Ca2+ alterations in familial Alzheimer's disease (FAD)
- Dr. Paola Pizzo
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34. Pancreatitis and calcium signaling
- Prof. Ole Petersen
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35. Mechanism-based therapies for heart failure and cardiac arrhythmias
- Prof. Andrew Marks
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36. Genetic defects and calcium
- Prof. Tullio Pozzan
- Archived Lectures *These may not cover the latest advances in the field
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37. Calcium, calmodulin and calcineurin
- Prof. Stephen Bolsover
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38. Calcium flickers steer cell migration
- Prof. Heping Cheng
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39. Automated Ca2+ imaging of chemosensory neurones in C.elegans
- Dr. Nikos Chronis
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40. Ca2+ and the regulation of small GTPases
- Prof. Peter Cullen
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41. Genetically encoded Ca2+ indicators: molecular scale measurements in mammals in vivo
- Dr. Michael I. Kotlikoff
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42. Capacitative (store-operated) calcium entry
- Dr. Jim Putney
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43. The molecular biology of the inositol trisphosphate receptor
- Dr. Randen Patterson
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44. Coordinated Ca2+ release from intracellular Ca2+ stores
- Prof. Ole Petersen
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45. The plasma membrane calcium pump: biochemistry, physiology and molecular pathology
- Prof. Ernesto Carafoli
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46. The calcium saga: a matter of life and death
- Prof. Pierluigi Nicotera
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47. Ca2+ efflux and Ca2+ signals
- Dr. Anne Green
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50. Modeling Ca2+ signals
- Dr. David Friel
Printable Handouts
Navigable Slide Index
- Introduction
- Importance of measuring Ca2+ concentrations
- Ca signaling and function in different cells
- Controlling Ca homeostasis
- Luminescent and fluorescent Ca probes
- Synthetic probes
- Fura-2/AM as a fluorescent Ca indicator dye
- The detection system
- Ca chelators derivatives: non-ratiometric probes
- Ca chelators derivatives: ratiometric probes
- Ratio (intensity) versus concentration
- Targeted Ca probes
- Measuring mitochondrial [Ca] with rhod-2
- Measuring organelle calcium
- Aequorin: a gene encoded Ca probe
- Recombinant probes
- Advantages of aequorin
- Aequorin activity as an indicator
- Reconstituting aequorin (protein+prosthetic group)
- Reconstituting aequorin where [Ca] is high
- Aequorin probes
- Using aequorin in population studies
- Measurement of [Ca] in the cytosole
- Measurement of [Ca] in the ER lumen
- Measurement of [Ca] in the mitochondria
- Disadvantages of aequorin (1)
- Disadvantages of aequorin (2)
- GFP-based probes
- Recombinant GFP-based fluorescent probes
- FRET:fluorescence resonance energy transfer
- Yellow cameleon
- Enhanced mitochondrial targeting of protein probes
- Camgaroo probe
- Ratiometric pericam probe
- New Ca sensors
Topics Covered
- Introduction to Calcium homeostasis
- Synthetic fluorescent Calcium probes
- Ratiometric and non-ratiometric probes
- Calibration
- Recombinant probes: the targeted probes
- Aequorin
- Targeting strategies
- Wide dynamic range
- Applications: some examples
- GFP-based probes
- Advantages and disadvantages
- Imaging
- FRET based probes
Talk Citation
Brini, M. (2007, October 1). Monitoring Ca2+ concentration in living cells [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved December 7, 2024, from https://doi.org/10.69645/SJPZ3035.Export Citation (RIS)
Publication History
Financial Disclosures
- Dr. Marisa Brini has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.