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Printable Handouts
Navigable Slide Index
- Introduction
- Valentino Gantz
- CRISPR/Cas9 technology
- CRISPR/Cas9 DNA break repair
- The mutagenic chain reaction (MCR)
- MCR conversion of sister allele
- Targeting yellow with an MCR
- Mendelian vs MCR inheritance
- Yellow-MCR inheritance
- The many facets of “Active Genetics”
- Malaria: Gene-drive strategies in mosquitoes
- Immunizing versus suppressing strategies
- Malaria: population modification
- Efficient gene-drive in flies and mosquitoes
- Logistic spread of an MCR element
- ERACR
- Drive versus ERACR
- CHACRs – updating platforms for gene-drives
- e-CHACR: an alternative ERACR strategy
- Trans-complementing MCR
- Active Genetics enabled by “CopyCat” elements
- Modular gene arrays using CopyCat elements (1)
- Modular gene arrays using CopyCat elements (2)
- Modular gene arrays using CopyCat elements (3)
- How to use in principle?
- The wing gene regulatory network (GRN)
- Using a double-cut CopyCat as a CHACR
- Using Copycat elements to compare function
- Safety considerations
- Credits
Topics Covered
- CRISPR/Cas9 technology
- The mutagenic chain reaction (MCR)
- Mendelian vs MCR inheritance
- Gene-drive strategies in mosquitoes
- Logistic spread of an MCR element
- ERACR gene-drive reversal elements
- CHACR elements
- e-CHACR as an alternative strategy
- Split-drive CopyCat elements
- Safety precautions used for gene-drive experiments
Talk Citation
Bier, E. (2018, April 30). The dawn of active genetics [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved October 5, 2024, from https://doi.org/10.69645/LKEH5952.Export Citation (RIS)
Publication History
Financial Disclosures
- Prof. Ethan Bier, Ownership Interest: Agragene and Synbal start-up companies
Other Talks in the Series: Gene-Drives and Active Genetics
Transcript
Please wait while the transcript is being prepared...
0:00
Hello. My name is Ethan Bier,
and today I'll be giving you a sense of some of
the interesting applications of active genetics in this talk that's entitled,
The Dawn of Active Genetics.
These two images you see by the way,
in front of you are the work of Valentino Gantz,
who in addition to being a fine scientist,
as you all hear, is also a very gifted artist.
0:29
So here is the gentleman himself, Valentino Gantz.
Again, the picture that he drew here of a fly,
where the wing that is out is indicating
a genetic process that repeats from
one generation to the next and the wing that is closed,
one that is part of the body,
where it's not happening.
It's a situation that works.
often, but not 100 percent of the time and he's likened
that to a little bit to a PCR machine which is embedded
in the thorax of that insect to give that kind of pictorial image of the process.
1:02
So, what Valentino had in mind was
a way of using what's known as the CRISPR/Cas9 technology,
and this is a technology that I imagine,
all of you are aware of.
It's a powerful revolutionary DNA editing technology that
allows for the directed cleavage of the DNA in the genome anywhere you want.
The way it works is that there's a guide RNA,
indicated gRNA on this slide,
that at the end of it has 20 nucleotides
that can be programmed to be any sequence you want.
To a first approximation then you can cut the genome in almost any location you want,
and what that does is,
that guide RNA combines with a protein that actually does the cutting called Cas9.
So, the Cas9 and the guide RNA together are this two component system that
allow you to cut as molecular scissors the genome in any location that you may want.
So, what Valentino came up with is a new way of using this molecular scissors.