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Comparing transcriptomes of distant organisms: models of gene expression 2
Published on August 31, 2017 22 min
A selection of talks on Cell Biology
Gene structure, expression and regulation: DNA structure and replication
- Dr. Carole Sargent
- University of Cambridge, UK
Preclinical translation of mesenchymal stem cell therapies
- Dr. Peter Childs
- University of Strathclyde, UK
In the last talk, we went over looking at the gene expression in detail and the comparative ENCODE resource, looking at non-coding transcription, looking at how things could be clustered. In this part, we're going to integrate gene expression with other types of activity on the genome, particularly activity related to the openness or closedness of the chromatin and also the activity of various regulatory factors binding to the DNA and turning things on and off. And we're going to try to make models that interrelate the openness to the chromatin, for instance, to the gene expression. We're also going to relate the factor binding to the gene expression in the framework of statistical models. And that's going to be these applications three and four, which I'll go on to right now.
So now we're going to move onto the third application, the building these models.
So the motivation is gene transcription, this is a fundamental process of gene transcription. Even though it's a very involved process, it sort of has to do with factors binding upstream of the gene, kind of opening up the chromatin, and the chromatin, as you think of it, sort of the DNA wrapped around these spools, and kind of opening it up, and then the gene being out read by a polymerase. And the open or closedness of the chromatin is often indicated by what's called histone modifications. And what we want to do, since we have a lot of data on the openness or closedness of the chromatin and the factor binding, we want to see if we can build a model that relates all these things together; And in building the model, it shows that we really understand the process.