• Overview of Molecular Chaperones
• 34 min
  1. History of the molecular chaperone concept: roles in assembly processes

  • Prof. Emeritus R. John Ellis
• 34 min
  2. Chaperone mechanisms in cellular protein folding

  • Prof. Dr. F. Ulrich Hartl
• Prokaryotic Molecular Chaperones
• 38 min
  3. Mechanistic aspects of chaperonin GroEL/ES function

  • Prof. Amnon Horovitz
• 99 min
  4. Structure and function of the ATP-dependent Clp chaperone/protease machines

  • Dr. Michael R. Maurizi
• 32 min
  5. The role of chaperones and Sec machinery in protein secretion

  • Prof. Koreaki Ito
• 44 min
  6. How can molecular chaperones repair damaged protein structures?

  • Prof. Pierre Goloubinoff
• 32 min
  7. Disulfide bond formation in vivo

  • Prof. James Bardwell
• Eukaryotic Molecular Chaperones
• 39 min
  8. Overview of eukaryotic molecular chaperones in the cytosol

  • Dr. Jason C. Young
• 36 min
  9. Chaperonin-containing TCP-1 (CCT), actin springs, and protein folding fluxes

  • Prof. Keith Willison
• 35 min
  10. The functions of the Hsp70 system

  • Prof. Jeffrey L. Brodsky
• 26 min
  11. Hsp90: a chaperone for protein kinases and hormone receptors

  • Dr. David Toft
• Role of Chaperones in Diseases
• 38 min
  12. The roles of molecular chaperones in bacterial infection

  • Prof. Tomoko Yamamoto
• 46 min
  13. Role of chaperonin-like proteins in Bardet-Biedl syndrome

  • Dr. Michel R. Leroux
• 53 min
  14. Roles for molecular chaperones in cystic fibrosis

  • Prof. Douglas M. Cyr
• 51 min
  15. Targeting cancer: designing drugs against Hsp90

  • Dr. Gabriela Chiosis
• Archived Lectures *These may not cover the latest advances in the field
• 29 min
  16. Overview of prokaryotic molecular chaperones

  • Prof. Walid A. Houry
• 47 min
  17. The biogenesis of E. coli inner membrane proteins

  • Dr. Joen Luirink
• 39 min
  18. Mechanism of chaperone action of small heat shock proteins

  • Prof. Elizabeth Vierling
• 22 min
  19. ClpB: a chaperone for protein disaggregation

  • Prof. Michal Zolkiewski
• 38 min
  20. The roles of chaperonins in bacteria

  • Dr. Peter Lund
• 44 min
  21. Towards a unifying mechanism for the Hsp70 chaperones

  • Prof. Pierre Goloubinoff
• 29 min
  22. Adaptor mediated activation of the Hsp100/ Clp protein ClpC controls general and regulatory proteolysis in Bacillus subtilis

  • Prof. Dr. Kursad Turgay
• 33 min
  23. Hsp31: a general stress protein of Escherichia coli

  • Prof. Francois Baneyx
• 46 min
  24. Hsp104: a specialized chaperone for protein disaggregation

  • Dr. John R. Glover
• 47 min
  25. Molecular chaperones and handling of abnormal proteins in neurodegenerative disorders

  • Prof. Michael Sherman
• 20 min
  26. The role of chaperones in Parkinson's disease

  • Dr. Konstanze F. Winklhofer

Printable Handouts

PDF

Navigable Slide Index

1. Introduction
2. The protein folding process
3. The cytoplasm is very crowded and viscous
4. Proteins are not very stable
5. Concept of a molecular chaperone
6. Chaperones in E. coli
7. Chaperones to be discussed
8. Chaperones and biosynthesis in E. coli
9. Trigger factor
10. Structure of E. coli trigger factor
11. Structure of E. coli TF bound to ribosome
12. The cradle model of TF function
13. The dynamic cycling model for TF function
14. The Hsp70/Hsp40 chaperone system
15. E. coli DnaK/DnaJ/GrpE structures
16. DnaK/DnaJ/GrpE functional cycle
17. Evidence for the K/J/E cycle from structural data
18. Motifs bound by DnaK and DnaJ
19. GroEL/GroES system
20. GroEL/GroES structure before and after binding
21. GroEL apical domain is implicated in binding
22. Mechanism used by GroEL in protein binding
23. GroEL cycle
24. GroEL function in vivo
25. The Clp ATPases
26. ClpB as a protein disaggregation machine
27. ClpXP complex model structure
28. Substrates of ClpXP
29. SsrA targets unfinished proteins for proteolysis
30. ClpXP mechanism of function
31. Model for substrate entry and product release
32. Conclusion

Topics Covered

• Protein folding in vitro versus in vivo
• Concept of a molecular chaperone
• Chaperones in E. coli
• Trigger factor
• DnaK/DnaJ/GrpE system
• GroEL/GroES system
• Clp ATPases

Links

Series:

• Molecular Chaperones

Categories:

• Biochemistry
• Cell Biology

Talk Citation

Publication History

• Published on October 1, 2007
• Archived on August 13, 2020

Financial Disclosures

• Prof. Walid A. Houry has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.