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1. Introduction
2. Fas ligand-induced cell death
3. Apoptosis-video
4. Activation of caspase
5. Signal transduction for Fas-mediated apoptosis
6. Apoptotic DNA fragmentation
7. CAD-mediated DNA degradation
8. Tertiary structure of CAD
9. No apoptotic DNA degradation in CAD-null cells
10. Apoptosis without DNA fragmentation
11. Multiple ways for caspase-mediated cell death
12. TUNEL-staining in vivo without CAD
13. Engulfment of apoptotic cells by macrophage
14. Two systems for apoptotic DNA degradation
15. Death of DNase II-null embryos in feto
16. Accumulation of undigested CAD-null nuclei
17. Accumulation of undigested nuclei in thymus
18. Long and intact DNA in lysosomes
19. Impaired thymic development in mutants
20. Induction of IFN-inducible genes
21. Induction of interferon beta gene
22. Rescue of the lethality of DNase II-null embryos
23. TLR mediated activation of IFNbeta gene
24. Non-involvement of TLRs system
25. Apoptosis and phagocytosis
26. Molecules for engulfment of apoptotic cells
27. Video - engulfment by a macrophage
28. Engulfment assay using CAD-/- cells
29. TUNEL assay for phagocytosis
30. Milk fat globule EGF factor 8
31. Binding of MFG-E8 and Del-1 to apoptotic cells
32. What is recognized by MFG-E8 and Del-1?
33. Specific binding MFG-E8 and Del-1 to PS
34. MFG-E8 and Del-1 work as a bridge
35. MFG-E8/Del-1-mediated engulfment
36. Expression of MFG-E8 in macrophages
37. Tingible-body macrophages
38. Inefficient engulfment of apoptotic cells
39. Inefficient ingestion of apoptotic cells
40. Splenomegaly in MFG-E8-deficient mice
41. Production of autoantibodies in MFG-E8-/- mice
42. Accumulation of immune complex
43. Model for the autoantibody production
44. Conclusions
45. Summary

Topics Covered

• Apoptosis
• DNA fragmentation during apoptosis
• DNA degradation in macrophages
• Activation of innate immunity by undigested DNA
• Engulfment of apoptotic cells
• Phosphatidylserine-dependent engulfment of apoptotic cells
• Autoimmune disease caused by unengulfed apoptotic cells

Links

Series:

• Apoptosis

Categories:

• Cell Biology

Therapeutic Areas:

• Immunology & Inflammation

Talk Citation

Publication History

• Published on October 1, 2007
• Archived on February 25, 2021

Financial Disclosures

• Prof. Shigekazu Nagata has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.